| 別品名 |
IL-1 beta, Interleukin-1 beta, IL-1β, catabolin
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| 種由来 |
Mouse
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Ion Exchange /Gel Filtration
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| 適用 |
Western Blot
Immunohistochemistry
Immuno Fluorescence
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| 免疫動物 |
Rabbit
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| 交差種 |
Mouse
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| GENE ID |
16176
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| Accession No.(Gene/Protein) |
CAA28637, P10749
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| Gene Symbol |
Il1b
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| 形状 |
凍結乾燥品
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| 参考文献 |
[Pub Med ID]32211925, 27090093, 32030468
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunofluorescence microscopy after staining of mouse carotid artery tissue with anti Mouse IL 1B antiserum (p/n 11 41 319, less purified form of 21 41 319) diluted 1:5. Tissue sections were prepared after cryofixation. Reaction occurred at room temperature for 6' followed by washes and reaction with Rhodamine conjugated Gt a Rabbit IgG (p/n 611 1 122). Tissue was counterstained with bis benzimide solution at .5 mg/ml in PBS for 15 min at room temperature. Panel A) shows no antibody staining of WT uninjured mouse carotid tissue. Panel B) shows anti IL 1B staining of cells after surgical injury of tissue. Panel C) shows no antibody staining of injured carotid tissue from an IL 1B KO mouse.
This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1s containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1s precursor molecule. Denatured IL-1s will have a 18 kDa band.
Immunohistochemistry of Rabbit anti-IL1Beta Antibody in Mouse Embryonic Kidney Tissue: Mouse Embryonic Kidney Fixation: FFPE buffered formalin 1% conc Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 2ug/ml 1.5 hour @ room T Secondary Ab: MACH 1 HRP POLYMER 1:5 45 RT
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Immunofluorescence microscopy after staining of mouse carotid artery tissue with anti Mouse IL 1B antiserum (p/n 11 41 319, less purified form of 21 41 319) diluted 1:5. Tissue sections were prepared after cryofixation. Reaction occurred at room temperature for 6' followed by washes and reaction with Rhodamine conjugated Gt a Rabbit IgG (p/n 611 1 122). Tissue was counterstained with bis benzimide solution at .5 mg/ml in PBS for 15 min at room temperature. Panel A) shows no antibody staining of WT uninjured mouse carotid tissue. Panel B) shows anti IL 1B staining of cells after surgical injury of tissue. Panel C) shows no antibody staining of injured carotid tissue from an IL 1B KO mouse.
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| メーカー |
品番 |
包装 |
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RKL
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210-401-319
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
４℃
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