| 別品名 |
IL-1 beta, Interleukin-1 beta, IL-1β, catabolin
|
| 抗原部位 |
N-terminus
|
| 種由来 |
Human
|
| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Ion Exchange /Gel Filtration
|
| 適用 |
Western Blot
|
| 免疫動物 |
Rabbit
|
| 交差種 |
Human
Canine
Primates
|
| GENE ID |
3553
|
| Accession No.(Gene/Protein) |
P01584
|
| Gene Symbol |
IL1B
|
| 形状 |
凍結乾燥品
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| 参考文献 |
Auron, P.E., A.C. Webb, et al (1984) Nucleotide sequence of human monocyte interleukin 1 precursor cDNA. Proc Natl Acad Sci USA 81: 7907-7911. Cerretti, D.P., C.J. Kozlosky, et al. (1992) Molecular cloning of the IL-1βprocessing enzyme. Science 256: 97-100. Herzyk, D.J., A.E. Berger, et al. (1992) Sandwich ELISA formats designed to detect 17 kDa IL-1βsignigicantly underestimate 35 kDa IL-1β J Immunol Meth 148: 243 -254.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot showing detection of recombinant human IL-1s by Rockland's anti-IL-1s antibody. Recombinant human IL-1s was loaded on to an SDS-PAGE gel at 0.1 μg and after separation, transferred to nitrocellulose. The expected band is approximately 18 kDa in size. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDocR 4000 MP imaging system.
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Western blot showing detection of recombinant human IL-1s by Rockland's anti-IL-1s antibody. Recombinant human IL-1s was loaded on to an SDS-PAGE gel at 0.1 μg and after separation, transferred to nitrocellulose. The expected band is approximately 18 kDa in size. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDocR 4000 MP imaging system.
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| メーカー |
品番 |
包装 |
|
RKL
|
209-401-B73
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100 UG
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
|
| 保存温度 |
４℃
|
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