| 励起波長 |
540
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| 蛍光波長 |
540
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| 検出系 |
発色/蛍光
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| 使用目的 |
本キットは、100μLのサンプル(100nM)中の10pgのNADまたはNADHをワンステップで高感度に検出する蛍光アッセイです。96ウェルまたは384ウェルマイクロプレートフォーマットで簡単に行えます。
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| 構成内容 |
・NADP/NADPH Recycling Enzyme Mixture 2 bottles (lyophilized powder)
・NADPH Sensor Buffer 1 bottle (20 mL)
・NADPHスタンダード 1 vial (167 μg)
・NADPH抽出溶液 1 bottle (10 mL)
・NADP抽出溶液 1 bottle (10 mL)
・NADP/NADPH Control Solution 1 bottle (10 mL)
・NADP/NADPH Lysis Buffer 1 bottle (10 mL)
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| 参考文献 |
Go, Jun and Seo, Ji Yeon and Park, Tae-Shin and Ryu, Young-Kyoung and Park, Hye-Yeon and Noh, Jung-Ran and Kim, Yong-Hoon and Hwang, Jung Hwan and Choi, Dong-Hee and Hwang, Dae Youn and others. (2018). Piperlongumine activates Sirtuin1 and improves cognitive function in a murine model of Alzheimera's disease. Journal of Functional Foods, 43, 103--111.
Hornsveld, Marten and Smits, Lydia M and Meerlo, Maaike and van Amersfoort, Miranda and Koerkamp, Marian J Groot and van Leenen, Dik and Kloet, David E and Holstege, Frank C and Derksen, Patrick WB and Burgering, Boudewijn MT and others. (2018). FOXO transcription factors both suppress and support breast cancer progression.. Cancer Research, , canres--2511.
Li, Miao and Liu, Xin and He, Yongpeng and Zheng, Qingyin and Wang, Min and Wu, Yu and Zhang, Yuanpeng and Wang, Chaoyun. (2017). Celastrol attenuates angiotensin II mediated human umbilical vein endothelial cells damage through activation of Nrf2/ERK1/2/Nox2 signal pathway. European Journal of Pharmacology, 797, 124--133.
Ling, Min and Huang, Peixin and Islam, Shamima and Heruth, Daniel P and Li, Xuanan and Zhang, Li Qin and Li, Ding-You and Hu, Zhaohui and Ye, Shui Qing. (2017). Epigenetic regulation of Runx2 transcription and osteoblast differentiation by nicotinamide phosphoribosyltransferase. Cell & Bioscience, 7(1), 27.
Luo, Gang and Huang, Bingqing and Qiu, Xiang and Xiao, Lin and Wang, Ning and Gao, Qin and Yang, Wei and Hao, Liping. (2017). Resveratrol attenuates excessive ethanol exposure induced insulin resistance in rats via improving NAD+/NADH ratio. Molecular Nutrition & Food Research, .
Ren, T and Zhang, H and Wang, J and Zhu, J and Jin, M and Wu, Y and Guo, X and Ji, L and Huang, Q and Yang, H and others. (2017). MCU-dependent mitochondrial Ca2+ inhibits NAD+/SIRT3/SOD2 pathway to promote ROS production and metastasis of HCC cells. Oncogene, .
Saihara, Kazuhiro and Kamikubo, Ryosuke and Ikemoto, Kazuto and Uchida, Koji and Akagawa, Mitsugu. (2017). Pyrroloquinoline Quinone, a Redox-active o-Quinone, Stimulates Mitochondrial Biogenesis by Activating SIRT1/PGC-1α Signaling Pathway. Biochemistry, .
Yang, Fei and Heit, Caitlin and Inglis, Debra L. (2017). Cytosolic Redox Status of Wine Yeast (Saccharomyces Cerevisiae) under Hyperosmotic Stress during Icewine Fermentation. Fermentation, 3(4), 61.
Zhang, Li Q and Van Haandel, Leon and Xiong, Min and Huang, Peixin and Heruth, Daniel P and Bi, Charlie and Gaedigk, Roger and Jiang, Xun and Li, Ding-You and Wyckoff, Gerald and others. (2017). Metabolic and molecular insights into an essential role of nicotinamide phosphoribosyltransferase. Cell Death & Disease, 8(3), e2705.
Ali, Yousuf O and Allen, Hunter M and Yu, Lei and Li-Kroeger, David and Bakhshizadehmahmoudi, Dena and Hatcher, Asante and McCabe, Cristin and Xu, Jishu and Bjorklund, Nicole and Taglialatela, Giulio and others. (2016). NMNAT2: HSP90 Complex Mediates Proteostasis in Proteinopathies. PLoS Biol, 14(6), e1002472.
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| 使用文献 |
Luo Hong-min, Wu Xia, Xian Xian, Wang Lu-yao, Zhu Liang-yu, Sun Hong-yu, Yang Lei, Liu Wen-xuan. (2020). Calcitonin gene-related peptide inhibits angiotensin II-induced NADPH oxidase-dependent ROS via the Src/STAT3 signalling pathway. Journal of Cellular and Molecular Medicine.
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| メーカー |
品番 |
包装 |
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ABD
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15264
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1 KIT
[250 Tests]
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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