Alomone Labs Recombinant human proBDNF (cleavage resistant) protein mediates?neurites outgrowth in TrkB transfected PC12 cells. - Cells were transiently transfected with TrkB/pcDNA3 containing the green fluorescence protein (GFP) as a reporter. One day post transfection, the cells were stimulated with 20 ng/ml Recombinant?human proBDNF (cleavage resistant) protein?(#B-256) or 10ng/ml?Recombinant human BDNF protein?(#B-250). Development of neurites was visualized after 6 days using bright light microscopy.