| 別品名 |
RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1
|
| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Serum
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Sheep
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| 交差種 |
Human
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| GENE ID |
207
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| Accession No.(Gene/Protein) |
P31749
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| Gene Symbol |
AKT1
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| 形状 |
滅菌済み液状品
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| 参考文献 |
Lawlor, M. A. and Alessi, D.R. (2001). PKB/AKT: a key mediator of cell proliferation, survival and insulin responses. J. Cell Science 114:2903-2910. Alessi, D. R. (2001). Discovery of PDK1, one of the missing links in insulin signal transduction. Biochem. Soc. Trans. 29,1 -14. Chu,S., Li,L., Singh,H. and Bhatia,R. (2007) BCR-tyrosine 177 plays an essential role in Ras and Akt activation and in human hematopoietic progenitor transformation in chronic myelogenous leukemia. Cancer Res. 67 (14), 7045-7053.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot of Anti AKT Antibody. Lane 1: HeLa cells (p/n W09 000 364). Lane 2: H2O2 treated MCF7 cells (p/n W09 000 366). Lane 3: Recombinant AKT. Lane 4: A431 cells (p/n W09 000 372). Load: 35 ug per lane. Primary antibody: AKT antibody at 1:300 for overnight at 4C. Secondary antibody: IRDYE800TM conjugated Dnky a Sheep IgG was used at a 1:10,000 dilution for 45 min at room temperature. Block: 1% BLOTTO (B501) overnight at 4C. Predicted/Observed size: ~55 60 kDa for AKT . Other band(s): unspecific.
Western Blot of Sheep Anti-AKT Antibody. Lane 1: Opal prestained Molecular weight markers (p/n MB-210-0500). Lane 2: rec. AKT1 (p/n 009-001-P21). Lane 3: rec. AKT2 (p/n 009-001-P22). Lane 4: rec. AKT3 (p/n 009-001-P23). Load: 50ng. Primary antibody: AKT1 antibody at 1:1000 for overnight at 4C. Secondary antibody: Donkey anti-Sheep IgG HRP (613-7302) at 1:70,000 for 30min at room temperature. Block: (p/n MB-073) BlockOut Buffer overnight at 4C. Predicted/Observed size: ~60 kDa for AKT.
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Western Blot of Anti AKT Antibody. Lane 1: HeLa cells (p/n W09 000 364). Lane 2: H2O2 treated MCF7 cells (p/n W09 000 366). Lane 3: Recombinant AKT. Lane 4: A431 cells (p/n W09 000 372). Load: 35 ug per lane. Primary antibody: AKT antibody at 1:300 for overnight at 4C. Secondary antibody: IRDYE800TM conjugated Dnky a Sheep IgG was used at a 1:10,000 dilution for 45 min at room temperature. Block: 1% BLOTTO (B501) overnight at 4C. Predicted/Observed size: ~55 60 kDa for AKT . Other band(s): unspecific.
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| メーカー |
品番 |
包装 |
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RKL
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600-601-401
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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