別品名 |
CASZ1, Zinc finger protein castor homolog 1, Castor-related protein, Zinc finger protein 693
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay
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免疫動物 |
Rabbit
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交差種 |
Human
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GENE ID |
54897
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Accession No.(Gene/Protein) |
Q86V15
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Gene Symbol |
CASZ1
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形状 |
滅菌済み液状品
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参考文献 |
[Pub Med ID]32060262, 21490919, 23592795, 27270431, 30899776
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot of Anti CASZ1 Antibody. Lane 1: NBLS Cytoplasmic (20ug). Lane 2: NBLS Nuclear (3ug). Lane 3: BE2C Cytoplasmic (30ug). Lane 4: BE2C Nuclear (7ug). Lane 5: SY5Y CASZ1b (10ug). Block: 5% Blotto/TTBS for 1 hour. Primary: Casz1 1:10,000 for 1 hour. Secondary: Goat anti Rabbit HRP for 1 hour. 240sec exposure. Detects nuclear endogenous CASZ1a and CASZ1b; and transiently transfected CASZ1b isoform. Personal communication and images from Carol Thiele Galetto, NCI.
Immunohistochemistry results of Rabbit Anti-hCASZ1 Antibody. Tissue: NB patient tumor. A. Score 0- a rare positive nuclei. B. Score 1- (1-10% positive) equivocal/uninterpretable. C. Score 2- (10-50% positive) weak positive. D. Score 3- (>50% positive) strong positive. Primary Antibody: Rabbit Anti-CASZ1 stained brown. Nucleus counterstained with hematoxylin (blue). Localization: Nuclear.
Immunohistochemistry results of Rabbit Anti-hCasz1 Antibody. Tissue: NB patient tumor. A. CASZ1 localized exclusively in the cytoplasm. B. CASZ1 localized in the cytoplasm and nucleus. Primary Antibody: Rabbit Anti-CASZ1 stained brown. Nucleus counterstained with hematoxylin (blue).
Immunofluorescence results of Endogenous CASZ1. Cells: BE2 cells. With or without Pre-Incubation of Anti-CASZ1 Antibody with CASZ1 Peptide. Staining: Rabbit Anti-CASZ1 Antibody. Chromatin counter stain: DAPI.
Immunofluorescence results of Rabbit Anti-CASZ1 Antibody. Tissue: Mouse Xenograft tumor of human NB cell line transfected with or without tetracycline inducible CASZ1 (NGPtetCASZ1a). Antibody: Rabbit Anti-CASZ1 Antibody. Counterstain: DAPI.
Immunofluorescence of Rabbit anti-CASZ1 Antibody. Tissue: adult murine ocular tissue. Antibody: Rabbit Anti-CASZ1 Antibody. Counterstain: DAPI. Localization: nucleus in lens epithelia but primarily localizes in the cytoplasm in photoreceptor cells.
Western blot using Rockland's anti-hCASZ1 antibody. This blot shows detection of endogenous and transfected human CASZ1 protein in fresh whole cell lysate (~30 ug). Lane 1: BE2(s) cell lysate. Lane 2: BE2(N) cell lysate. Lane 3: SY5Y transfected with hCASZ5 (125kDa). Lane 4: SY5Y transfected with hCASZ11 (190kDa). Protein was resolved by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000 for 1.5 hours at room temperature then incubated with HRP-conjugated Goat Anti-Rabbit antibody for 45 min. at room temperature. Personal communication, Carol Thiele, NCI, Bethesda, MD.
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Western Blot of Anti CASZ1 Antibody. Lane 1: NBLS Cytoplasmic (20ug). Lane 2: NBLS Nuclear (3ug). Lane 3: BE2C Cytoplasmic (30ug). Lane 4: BE2C Nuclear (7ug). Lane 5: SY5Y CASZ1b (10ug). Block: 5% Blotto/TTBS for 1 hour. Primary: Casz1 1:10,000 for 1 hour. Secondary: Goat anti Rabbit HRP for 1 hour. 240sec exposure. Detects nuclear endogenous CASZ1a and CASZ1b; and transiently transfected CASZ1b isoform. Personal communication and images from Carol Thiele Galetto, NCI.
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メーカー |
品番 |
包装 |
RKL
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600-401-B62S
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25 UL
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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