別品名 |
p90 RSK1, Ribosomal S6 Kinase 1, RSK1, S6K-alpha 1, 90 kDa Ribosomal Protein S6 Kinase 1, MAP kinase-activated protein kinase 1a, or MAPKAPK1A
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抗原部位 |
C-terminus
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay
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免疫動物 |
Rabbit
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交差種 |
Human
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翻訳後修飾 |
リン酸化
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GENE ID |
6195
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Accession No.(Gene/Protein) |
NP_001006666, Q15418
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Gene Symbol |
RPS6KA1
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形状 |
滅菌済み液状品
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参考文献 |
[Pub Med ID]24307699
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot of Rabbit anti p90 RSK1 antibody. Lane 1: unstimulated HEK293T cell lysates. Lane 2: EGF stimulated HEK293T cell lysates. Load: 35 ug per lane.Primary antibody: p90 RSK1 antibody at 1:1000 for overnight at 4C.Secondary antibody: peroxidase conjugated secondary antibody and ECL.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: 90 kDa, ~90 kDa for p90 RSK1. Other band(s): none.
Western blot using Rockland's affinity purified anti-p90 RSK1 pS732 antibody shows detection of a band ~90 kDa in size corresponding to phosphorylated p90 RSK1 (arrowhead) in EGF stimulated (lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (lane 1). After transfer, the membrane was blocked overnight followed by reaction with the primary antibody at a 1:1,000 dilution. Detection occurred using a peroxidase conjugated secondary antibody and ECL. Personal Communication. Kuldeep Patel, Loyola University Medical Center, Maywood, IL.
Western blot using Rockland's affinity purified anti-p90 RSK1 pS732 antibody. Lane 1-2: HEK293T (U) untreated or (T) treated with EGF. Lane 3-4: HEK293T (U) untreated or (T) treated with EGF. Lane 5-6: HEK293T (U) untreated or (T) treated with EGF. Load: 15ug per lane. Actin used as a loading control. Blocking: 5% milk. Primary Antibody: Anti-RSK1-pS732 1:1000, 1:2000, or 1:3000 O/N. Secondary Antibody: Goat Anti-Rabbit IgG 1:5000 for 2 hours. Predicted Size: ~90 kDa in size corresponding to phosphorylated p90 RSK1 in EGF stimulated. Personal Communication. Kuldeep Patel, Loyola University Medical Center, Maywood, IL.
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Western Blot of Rabbit anti p90 RSK1 antibody. Lane 1: unstimulated HEK293T cell lysates. Lane 2: EGF stimulated HEK293T cell lysates. Load: 35 ug per lane.Primary antibody: p90 RSK1 antibody at 1:1000 for overnight at 4C.Secondary antibody: peroxidase conjugated secondary antibody and ECL.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: 90 kDa, ~90 kDa for p90 RSK1. Other band(s): none.
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メーカー |
品番 |
包装 |
RKL
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600-401-B30S
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25 UL
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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