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Immunohistochemistry of rabbit anti-NCOA3 antibody. Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-NCOA3 at 5 μg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.
Immunohistochemistry of rabbit anti-NCOA3 antibody. Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-NCOA3 at 5 μg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.
Western blot using Rockland's affinity purified anti-NCOA3 antibody shows detection of NCOA3 in mouse liver nuclear extract (lane 1), transient transfected 293 cell lysate (lane 2), HeLa whole cell lysate (lane 3) and mouse thyroid cell nuclear extract (lane 4). The band at ~148 kDa, indicated by the arrowhead, corresponds to NCOA3. Mouse NCOA3 (lanes 1 and 4) appear as ~105 kDa bands. Pre-incubation of antibody with the immunizing peptide blocks detection of specific band staining (not shown). Personal communication, H. Ying, NCI, Bethesda, MD.
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Immunohistochemistry of rabbit anti-NCOA3 antibody. Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-NCOA3 at 5 μg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.
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| 別品名 |
rabbit anti-NCOA3 antibody, NCOA 3, NCOA-3, Nuclear receptor coactivator 3 NCoA-3, Thyroid hormone receptor activator molecule 1, TRAM-1, pCIP, bHLHe42, Receptor-associated coactivator 3, RAC-3, Amplified in breast cancer 1 protein, AIB-1, Steroid receptor coactivator protein 3, SRC-3, ACTR, AIB1, BHLHE42, RAC3, TRAM1
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
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| 免疫動物 |
Rabbit
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| GENE ID |
8202
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| Accession No.(Gene/Protein) |
32307126, Q9Y6Q9
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| Gene Symbol |
NCOA3
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| 参考文献 |
Li,H.J., Haque,Z., Lu,Q., Li,L., Karas,R. and Mendelsohn,M.(2007) Steroid receptor coactivator 3 is a coactivator for myocardin, the regulator of smooth muscle transcription and differentiation. Proc. Natl. Acad. Sci. U.S.A. 104 (10), 4065-4070. Naeem,H., Cheng,D., Zhao,Q., Underhill,C., Tini,M., Bedford,M.T. and Torchia,J. (2007) The activity and stability of the transcriptional coactivator p/CIP/SRC-3 are regulated by CARM1-dependent methylation. Mol. Cell. Biol. 27 (1), 120-134. Hossain,A., Kuo,M.T. and Saunders,G.F. (2006) Mir-17-5p regulates breast cancer cell proliferation by inhibiting translation of AIB1 mRNA. Mol. Cell. Biol. 26 (21), 8191-8201.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-A40S
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25 UL
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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