| 別品名 |
Interleukin-33, Interleukin-1 family member 11, IL-1F11, nuclear factor from high endothelial venules and NF-HEV
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Purified
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| 適用 |
Western Blot
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| 免疫動物 |
Rabbit
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| 交差種 |
Human
|
| GENE ID |
90865
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| Accession No.(Gene/Protein) |
O95760
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| Gene Symbol |
IL33
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| 形状 |
滅菌済み液状品
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| 参考文献 |
Hayakawa,M., Hayakawa,H., Matsuyama,Y., Tamemoto,H., Okazaki,H. and Tominaga,S. (2009) Mature interleukin-33 is produced by calpain-mediated cleavage in vivo. Biochem. Biophys. Res. Commun. 387 (1), 218-222. Luthi,A.U., Cullen,S.P., McNeela,E.A., Duriez,P.J., Afonina,I.S., Sheridan,C., Brumatti,G., Taylor,R.C., Kersse,K., Vandenabeele,P., Lavelle,E.C. and Martin,S.J. (2009) Suppression of interleukin-33 bioactivity through proteolysis by apoptotic caspases. Immunity 31 (1), 84-98. Wood,I.S., Wang,B. and Trayhurn,P. (2009) IL-33, a recently identified interleukin-1 gene family member, is expressed in human adipocytes. Biochem. Biophys. Res. Commun. 384 (1), 105-109. Pushparaj,P.N., Tay,H.K., H'ng,S.C., Pitman,N., Xu,D., McKenzie,A., Liew,F.Y. and Melendez,A.J. (2009) The cytokine interleukin-33 mediates anaphylactic shock. Proc. Natl. Acad. Sci. U.S.A. 106 (24), 9773-9778.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot using Rockland's anti-Human IL-33 antibody shows detection of a band ~18 kDa in size corresponding to recombinant human IL-33 (lane 1). The identity of the higher molecular weight band is unknown. Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax？ chemiluminescent substrate. Image was captured using VersaDoc？ MP 4000 imaging system (Bio-Rad).
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Western blot using Rockland's anti-Human IL-33 antibody shows detection of a band ~18 kDa in size corresponding to recombinant human IL-33 (lane 1). The identity of the higher molecular weight band is unknown. Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax？ chemiluminescent substrate. Image was captured using VersaDoc？ MP 4000 imaging system (Bio-Rad).
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| メーカー |
品番 |
包装 |
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RKL
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209-401-B50S
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25 UL
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※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
|
| 保存温度 |
-２０℃
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