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Western blot using Rockland's anti-Human IL17-A antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG (p/n 611-143-122) secondary antibody diluted 1:20,000 in blocking buffer (p/n MB-070). Image was captured using VersaDoc？ MP 4000 imaging system (Bio-Rad).
Immunohistochemistry of Rabbit Anti-human IL-17A Antibody. Tissue A: Human Small intestine H&E. Tissue B: IL-17A in Small intestine (20X). Tissue C: IL-17A in Small intestine (40X). Antigen Retrieval: HIER using Citrate Buffer for 20 min. Primary Antibody: anti-IL-17A at 1:100 at RT for 30min. Secondary Antibody: Ready To Use Anti-Rabbit Poly-HRP IgG at RT for 8 min. Staining: DAB. Counter Stain: Hematoxylin.
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Western blot using Rockland's anti-Human IL17-A antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG (p/n 611-143-122) secondary antibody diluted 1:20,000 in blocking buffer (p/n MB-070). Image was captured using VersaDoc？ MP 4000 imaging system (Bio-Rad).
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| 別品名 |
rabbit anti-Interleukin-17A antibody, rabbit anti-IL-17A antibody, IL-17A, Interleukin-17A, Cytotoxic T-Lymphocyte-associated Antigen 8, CTLA8, Interleukin-17, IL17, IL-17, Interleukin17
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| 交差種 |
Human
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| 適用 |
Western Blot
Immunohistochemistry
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| 免疫動物 |
Rabbit
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| GENE ID |
3605
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| Accession No.(Gene/Protein) |
AAH662531.1, Q16552
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| Gene Symbol |
IL17A
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| 参考文献 |
Shin,J.H., Shin,D.W. and Noh,M. (2009) Interleukin-17A inhibits adipocyte differentiation in human mesenchymal stem cells and regulates pro-inflammatory responses in adipocytes. Biochem. Pharmacol. 77 (12), 1835-1844. Yin,Y., Wen,S., Li,G. and Wang,D. (2009) Hypoxia enhances stimulating effect of amyloid beta peptide (25-35) for interleukin 17 and T helper lymphocyte subtype 17 upregulation in cultured peripheral blood mononuclear cells. Microbiol. Immunol. 53 (5), 281-286. Paust,H.J., Turner,J.E., Steinmetz,O.M., Peters,A., Heymann,F., Holscher,C., Wolf,G., Kurts,C., Mittrucker,H.W., Stahl,R.A. and Panzer,U. (2009) The IL-23/Th17 axis contributes to renal injury in experimental glomerulonephritis. J. Am. Soc. Nephrol. 20 (5), 969-979.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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209-401-B32S
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25 UL
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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