Anti TRPC6  

Immunohistochemistry using Rockland's anti-TRPC6 monoclonal antibody shows detection of TRPC6 in human adrenal (cortex) tissue (40X). The antibody was used a dilution to 2.5 μg/mL. The image shows strong staining with minimal background staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.

Western Blot of Mouse Anti-TRPC6 Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: Mouse Pancreas Tissue Lysate (p/n W10-000-T023) [10μg]. Lane 3: MCF-7 Whole Cell Lysate (p/n W09-000-360) [10μg]. Lane 4: A431 Whole Cell Lysate (p/n W09-000-361) [10μg]. Lane 5: Jurkat Whole Cell Lysate (p/n W09-001-370) [10μg]. Primary Antibody: Anti-TRPC6 at 1μg/mL overnight at 2-8°C. Secondary Antibody: Rabbit Anti-Mouse IgG Peroxidase (p/n 610-403-C46) 1:40000 for 30mins at RT. Blocking Buffer: BlockOut Buffer (p/n MB-073) for 30mins at RT. Predicted MW: ~30kDa. Observed MW: ~40kDa. Exposure: 5sec.

ELISA Results of Mouse Anti-TRPC6 Antibody. Each well was coated in duplicate with 0.1μg of conjugate. The working dilution is 1:31,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP conjugation Stabilizer (p/n MB-076), Rabbit Anti-Mouse IgG HRP conjugated (p/n 610-403-C46) and TMB substrate (p/n TMBE-1000).

Western Blot of Mouse anti-TRPC6 Antibody. Lane 1: Mouse Kidney WCL (p/n W10-000-T017). Load: 10 μg per lane. Primary antibody: TRPC6 Antibody at 1:1000 for overnight at 4°C. Secondary antibody: donkey anti-mouse DyLight^TM 649 (p/n 610-743-002) at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT.

Immunohistochemical localization TRPC6 using anti-TRPC6 monoclonal (A,B) and polyclonal (C) antibodies in human digital Meissner corpuscles (around 22%). Immunoreactivity was found in axons supplying Meissner corpuscles and isolated thick nerve fibers (arrows). The pattern of TRPC6 immunolabelling (E？and？H; red and green fluorescence, respectively), matches the distribution of NFP (G; red fluorescence) but not of S100 protein (D, green fluorescence). When the images were overlapped there was colocalization of TRPC6 and NFP (I, merge) but not colocalization of TRPC6 and S100 protein (F, merge). Objective 40×/1.25 Oil; pinhole airy 1, XY resolution 156 nm and Z resolution 334 nm. e: epidermis. Fig 1. PMID:？27874267

Confocal laser-scanning images of TRPV4 (green fluorescence,？A) and TRPC6 (red,？B) in one single digital Meissner corpuscle. Figure？C？is a merge image of TRPV4 and TRPC6 covered by mask (D, white dots). Mask represents the regions of interest drawn on the cytofluorogram around the high red signal dots. Images？E？and？F？show the quantification of fluorescente intensity of images 3A and 3B, respectively. The values range from 0 (green pixels) to 255 (blue pixels and the absence of blue pixels indicate that the images are unsaturated. Image in g shows a 2D cytofluorogram from the two detection channels from the original image. Every dot of cytofluorogram represents an intensity value pair from the two detection channels. Red signal dots are not close to the Y-axis because the green signal is widely distributed (including epidermis) and has been captured with higher gain amplification than those for the red signal. The area of interest surrounding the high red signal dots on cytofluorogram had been drawn and, on the merge image, is represented as a mask (white dots). e: epidermis. Fig 4.PMID:？27874267