別品名 |
TRPC6, TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6
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抗原部位 |
C-terminus
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種由来 |
Human
|
標識物 |
Unlabeled
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精製度 |
Ig fraction - Protein A
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry
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免疫動物 |
Mouse
|
抗体クラス |
IgG1κ
|
クローン |
3F2.H10.F2
|
交差種 |
Human Mouse
|
GENE ID |
7225
|
Accession No.(Gene/Protein) |
Q9Y210
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Gene Symbol |
TRPC6
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形状 |
滅菌済み液状品
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参考文献 |
[Pub Med ID]27874267
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunohistochemistry using Rockland's anti TRPC6 monoclonal antibody shows detection of TRPC6 in human adrenal (cortex) tissue (40X). The antibody was used a dilution to 2.5 ug/mL. The image shows strong staining with minimal background staining. Tissue was formalin fixed and paraffin embedded. No pre treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.
ELISA Results of Mouse Anti-TRPC6 Antibody. Each well was coated in duplicate with 0.1ug of conjugate. The working dilution is 1:31,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP conjugation Stabilizer (p/n MB-076), Rabbit Anti-Mouse IgG HRP conjugated (p/n 610-403-C46) and TMB substrate (p/n TMBE-1000).
Western Blot of Mouse Anti-TRPC6 Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: Mouse Pancreas Tissue Lysate (p/n W10-000-T023) [10ug]. Lane 3: MCF-7 Whole Cell Lysate (p/n W09-000-360) [10ug]. Lane 4: A431 Whole Cell Lysate (p/n W09-000-361) [10ug]. Lane 5: Jurkat Whole Cell Lysate (p/n W09-001-370) [10ug]. Primary Antibody: Anti-TRPC6 at 1ug/mL overnight at 2-8C. Secondary Antibody: Rabbit Anti-Mouse IgG Peroxidase (p/n 610-403-C46) 1:40000 for 30mins at RT. Blocking Buffer: BlockOut Buffer (p/n MB-073) for 30mins at RT. Predicted MW: ~30kDa. Observed MW: ~40kDa. Exposure: 5sec.
Western Blot of Mouse anti-TRPC6 Antibody. Lane 1: Mouse Kidney WCL (p/n W10-000-T017). Load: 10 ug per lane. Primary antibody: TRPC6 Antibody at 1:1000 for overnight at 4C. Secondary antibody: donkey anti-mouse DyLightTM 649 (p/n 610-743-002) at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT.
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Immunohistochemistry using Rockland's anti TRPC6 monoclonal antibody shows detection of TRPC6 in human adrenal (cortex) tissue (40X). The antibody was used a dilution to 2.5 ug/mL. The image shows strong staining with minimal background staining. Tissue was formalin fixed and paraffin embedded. No pre treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.
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メーカー |
品番 |
包装 |
RKL
|
200-301-B59S
|
25 UL
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※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
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保存温度 |
-20℃
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