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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot using Rockland's affinity purified anti-TRAF2 antibody shows detection of endogenous TRAF2 at ~47kDa (arrowhead). Lane 1 and 3: molecular weight markers. Lane 2: whole HeLa cell lysates (p/n W09-000-364). The identity of lower molecular weight band in lane 2 is unknown. Lane 4: incubated with immunizing peptide. Briefly, each lane contains approximately 14μg of lysate. Membranes were blocked in 3% BSA-TBS 30 min. at room temperature. Primary antibody was used at a 1:500 dilution in 3% BSA-TBS and reacted overnight at 4°C. The membrane was washed and reacted with a 1:20,000 dilution conjugated Gt-a-Rabbit DyLight 649 (p/n 611-143-122) for 1 hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers in lanes 1 and 3. Fluorescence image was captured using the VersaDocR Imaging System developed by Bio-Rad.
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Western blot using Rockland's affinity purified anti-TRAF2 antibody shows detection of endogenous TRAF2 at ~47kDa (arrowhead). Lane 1 and 3: molecular weight markers. Lane 2: whole HeLa cell lysates (p/n W09-000-364). The identity of lower molecular weight band in lane 2 is unknown. Lane 4: incubated with immunizing peptide. Briefly, each lane contains approximately 14μg of lysate. Membranes were blocked in 3% BSA-TBS 30 min. at room temperature. Primary antibody was used at a 1:500 dilution in 3% BSA-TBS and reacted overnight at 4°C. The membrane was washed and reacted with a 1:20,000 dilution conjugated Gt-a-Rabbit DyLight 649 (p/n 611-143-122) for 1 hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers in lanes 1 and 3. Fluorescence image was captured using the VersaDocR Imaging System developed by Bio-Rad.
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| 別品名 |
rabbit anti-TRAF2 antibody, E3 ubiquitin-protein ligase TRAF2, RING-type E3 ubiquitin transferase TRAF2, TNF receptor associated factor 2 antibody, TRAF-2, TRAF 2 antibody, TRAP-3, TRAP 3 antibody, TRAP3 antibody, Tumor necrosis factor type 2 receptor associated protein 3 antibody
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| GENE ID |
7186
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| Accession No.(Gene/Protein) |
22027612, Q12933
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| Gene Symbol |
TRAF2
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| 参考文献 |
Li S., Wang L., Berman M., Zhang Y., and Dorf M. (2006) RNAi Screen in Mouse Astrocytes Identifies Phosphatases that Regulate NF-κB Signaling. Mol Cell. Nov 17; 24:497-509.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-B27S
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25 UL
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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