| 別品名 |
SUMO E1 activating enzyme Ubiquitin-like 1 activating enzyme E1A UBLE1A AOS1 SAE1 SUA1
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 交差種 |
Human
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| 翻訳後修飾 |
リン酸化
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| GENE ID |
10055
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| Accession No.(Gene/Protein) |
NP_005491, Q9UBE0
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| Gene Symbol |
SAE1
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| 形状 |
滅菌済み液状品
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| 参考文献 |
Wrighton KH,and Feng XH. (2006). Uba2. AfCS-Nature Molecule Pages. doi:10.1038/mp.a003681.01 Lois,L.M. and Lima,C.D. (2005) Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1. EMBO J. 24 (3), 439-451 Desterro,J.M., Rodriguez,M.S., Kemp,G.D. and Hay,R.T. (1999) Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1. J. Biol. Chem. 274 (15), 10618-10624. Gong,L., Li,B., Millas,S. and Yeh,E.T. (1999) Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex. FEBS Lett. 448 (1), 185-189.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot using Rockland's Rabbit anti-SAE1 pS185 antibody shows detection of phosphorylated SAE1. Left lane (-) contains 20 μg human HeLa whole cell protein. Right lane (+) contains 20 μg human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephosphorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 1:1,000 at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX
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Western blot using Rockland's Rabbit anti-SAE1 pS185 antibody shows detection of phosphorylated SAE1. Left lane (-) contains 20 μg human HeLa whole cell protein. Right lane (+) contains 20 μg human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephosphorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 1:1,000 at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX
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| メーカー |
品番 |
包装 |
|
RKL
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600-401-B24S
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25 UL
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※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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