Anti Plasminogen Activator Inhibitor 2  

Immunohistochemistry of rabbit anti-Plasminogen Activator 2 antibody. Tissue: heart. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-Plasminogen Activator 2 at 10 μg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Plasminogen Activator II as precipitated red signal with hematoxylin purple nuclear counterstain.

Western blot using Rockland's affinity purified anti-PAI-2 antibody shows detection of endogenous PAI-2 in 50μg of HeLa whole cell lysates (p/n W09-000-364). The band at ~42kDa (arrowhead) corresponds to PAI-2. Faint non-specific bands are also noted at lower and higher molecular weights positions. Primary antibody was used at a 1:500 dilution in 5% BLOTTO (p/n B501-0500) in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1:5,000 dilution of HRP-conjugated Gt-a-Rabbit IgG (p/n 611-103-122) for 45 min at room temperature with ECL used for detection. Molecular weight estimation was made by comparison to prestained MW markers. Personal communication, Luca D’Agostino, Temple University, Philadelphia, PA.