Anti CENP-Q

Immunofluorescence microscopy using Rockland's protein A purified anti CENP Q antibody shows detection of endogenous CENP Q in HeLa whole cell lysate. Primary antibody was used at 1:100 followed by secondary antibody diluted 1:150. Red punctate anti CENP Q signal colocalizes in overlay images with green punctate anti CREST signals at the kinetochores (attached points of sister chromatids). Visible are colocalized CENP Q and CREST signal at various stages of the cell cycle as indicated from interphase to the end of mitosis. Nuclei are counter stained with bisbenzimide. Personal Communication, Kyung S. Lee, CCR NCI, Bethesda, MD

Western blot using Rockland's protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in a HeLa whole cell lysate (lane 1, arrowhead). The blot was incubated for 1.5 hours at room temperature using the primary antibody diluted to 0.5ug/mL, followed by washes and incubation with to the secondary antibody. Lane 1: Lysates from HeLa cells transfected with control sh-virus wherein the expression of CENP-Q is expected to not to alter. Lane 2: Lysates from HeLa cells transfected with Cenp-Q sh-virus wherein the expression of CENP-Q is knocked down significantly to a level where it is not being detected at all under the tested condition/WB exposure time. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.