別品名 |
RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1
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種由来 |
Human
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標識物 |
DyLightTM 549
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精製度 |
Ig fraction - Protein A
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適用 |
Western Blot
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免疫動物 |
Mouse
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抗体クラス |
IgG1κ
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クローン |
18F3.H11
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交差種 |
Human Mouse Rat Monkey
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翻訳後修飾 |
リン酸化
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GENE ID |
207
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Accession No.(Gene/Protein) |
P31749
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Gene Symbol |
AKT1
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形状 |
凍結乾燥品
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
DyLightTM dyes can be used for two color western blot detection with low background and high signal. Anti tubulin was detected using a DyLightTM 549 conjugate. Anti TNFa was detected using a DyLightTM 649 conjugate. The image was captured using the TyphoonTM 9410 Imaging System.
Properties of DyLightTM Fluorescent Dyes.
Immunohistochemistry of Mouse anti-AKT pT308 antibody. Tissue: human brain cerebellum tissue (40X). Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT pT308antibody at 20 μg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: staining of Purkinje neurons and cell processes in the cerebellum, cytosolic as well as occasionally nuclear. Staining: AKT pT308 as precipitated red signal with hematoxylin purple nuclear counterstain.
DyLightTM 549 Fluorescence absorption and emission spectra.
Western Blot of Mouse anti-AKT pT308 antibody DyLight 549 Conjugated. Lane 1: GST-AKT1 Unactive recombinant protein. Lane 2: GST-AKT1 Active recombinant protein. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: DyLight 549 conjugated Ms-a-AKT pT308 (p/n 200-342-269) was used at a 1:1,000 dilution for 1 h at RT. Block: 3% BSA (p/n BSA-30) in TBS for 30 min at RT. Predicted/Observed size: (indicated by arrowhead at ~79 kDa). Other band(s): None.
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DyLightTM dyes can be used for two color western blot detection with low background and high signal. Anti tubulin was detected using a DyLightTM 549 conjugate. Anti TNFa was detected using a DyLightTM 649 conjugate. The image was captured using the TyphoonTM 9410 Imaging System.
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メーカー |
品番 |
包装 |
RKL
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200-342-269
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100 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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法規制 |
毒
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保存温度 |
4℃
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