Green Fluorescent Protein (GFP) Control Protein

Western Blot of anti GFP monoclonal antibody. Lane 1: 64pg of recombinant GFP protein (p/n 000 001 215) were spiked into a HeLa cell derived lysates (p/n W09 000 364). Lane 2: 32pg of recombinant GFP protein were spiked into a HeLa cell derived lysates. Lane 3: 16pg of recombinant GFP protein were spiked into a HeLa cell derived lysates. Lane 4: 8pg of recombinant GFP protein were spiked into a HeLa cell derived lysates. Lane 5: 4pg of recombinant GFP protein were spiked into a HeLa cell derived lysates. Lane 6: 2pg of recombinant GFP protein were spiked into a HeLa cell derived lysates. Lane 7: 1g of recombinant GFP protein were spiked into a HeLa cell derived lysates. Lane 8: 0pg of recombinant GFP protein were spiked into a HeLa cell derived lysates. Primary antibody: anti GFP monoclonal antibody at 1:400 for overnight at 4C. Secondary antibody: HRP conjugated anti Mouse IgG (p/n 610 4302) was performed at a dilution of 1:20,000 for 1h at 4C. Block: TTBS (p/n MB 013) supplemented with 1% BSA (p/n BSA 50) for 1 h at 4C. Predicted/Observed size: 27 kDa for GFP. Other band(s): none.