別品名 |
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
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種由来 |
Human
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適用 |
Western Blot IHC paraffin embedding section Enzyme Linked Immunosorbent Assay
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免疫動物 |
Mouse
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抗体クラス |
IgG1κ
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クローン |
17F6.B11
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交差種 |
Human Mouse Rat Chimpanzee
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翻訳後修飾 |
リン酸化
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GENE ID |
207
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Accession No.(Gene/Protein) |
P31749;AAH00479
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Gene Symbol |
AKT1
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形状 |
液状
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参考文献 |
Jones PF, Jakubowicz T, Pitossi FJ, Maurer F, Hemmings BA. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4171-5. Alessi DR. Biochem Soc Trans. 2001 May;29(Pt 2):1-14. Lawlor MA, Alessi DR. J Cell Sci. 2001 Aug;114(Pt 16):2903-10.
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使用文献 |
[Pub Med ID]1851997, 11356119, 11686294
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot using AKT1 (phospho S473) monoclonal antibody, clone 17F6.B11 (Cat # MAB1919) shows detection of phospho-AKT1 (indicated by arrowhead at ~56 KDa) on PDGF stimulated NIH/3T3 cell lysates (Lane 2). No reactivity is seen for non-phosphorylated AKT1 in untreated cells (Lane 1). Each lane contained approximately 10 ug of lysate. All samples were loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 5% BLOTTO in TBS for 90 min at RT. The membrane was probed with the primary antibody at a 1:10,000 dilution in TBS with 0.05% Tween-20 with 1% BSA, for 1 h at 4°C. For detection HRP conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 1 h at 4°C with FemtoMax™ enhanced chemiluminescent reagent. Images were captured using 2X2 binning for 10-20 sec using a BioSpectrum Imaging System (UVP Ltd.).
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|
Western blot using AKT1 (phospho S473) monoclonal antibody, clone 17F6.B11 (Cat # MAB1919) shows detection of phospho-AKT1 (indicated by arrowhead at ~56 KDa) on PDGF stimulated NIH/3T3 cell lysates (Lane 2). No reactivity is seen for non-phosphorylated AKT1 in untreated cells (Lane 1). Each lane contained approximately 10 ug of lysate. All samples were loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 5% BLOTTO in TBS for 90 min at RT. The membrane was probed with the primary antibody at a 1:10,000 dilution in TBS with 0.05% Tween-20 with 1% BSA, for 1 h at 4°C. For detection HRP conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 1 h at 4°C with FemtoMax™ enhanced chemiluminescent reagent. Images were captured using 2X2 binning for 10-20 sec using a BioSpectrum Imaging System (UVP Ltd.).
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メーカー |
品番 |
包装 |
ABV
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MAB1919
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100 UG
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※表示価格について
当社在庫 |
なし
|
納期目安 |
2週間程度
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保存温度 |
-20℃
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