別品名 |
DyLight Dye, DyLight Conjugated Antibody
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種由来 |
Rat
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標識物 |
DyLightTM 649
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精製度 |
Affinity Purified
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適用 |
Western Blot
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免疫動物 |
Rabbit
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交差種 |
Rat
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形状 |
凍結乾燥品
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
DyLightTM dyes can be used for two color Western Blot detection with low background and high signal. Anti tubulin was detected using a DyLightTM 549 conjugate. Anti TNFa was detected using a DyLightTM 649 conjugate. The image was captured using the TyphoonTM 9410 Imaging System.
Western Blot of Anti-Rat IgG (H&L) (RABBIT) Antibody (p/n 612-4102). Lane M: 3 μl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (RABBIT) Antibody (p/n 612-4102) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
DyLightTM 649 Fluorescence Spectra.
Properties of DyLightTM Fluorescent Dyes.
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DyLightTM dyes can be used for two color Western Blot detection with low background and high signal. Anti tubulin was detected using a DyLightTM 549 conjugate. Anti TNFa was detected using a DyLightTM 649 conjugate. The image was captured using the TyphoonTM 9410 Imaging System.
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メーカー |
品番 |
包装 |
RKL
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612-443-002
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100 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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法規制 |
毒
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保存温度 |
4℃
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