Anti IgG (H&L)  
DyLight (商標名)

Detection of newly synthesized proteins by puromycilation.？(A)？Rat hippocampal neurons were grown for 9 DIV and were treated with DMSO (left panels) or Aβ1？42？oligomers (right panels) for 24 h. Before fixing, cells were incubated with vehicle (-puro; neurites 1 and 2), with puromycin (+ puro; neurites 3 and 4) or with puromycin and anisomycin (+ anis + puro; neurites 5 and 6) for 30 mins. Cells were immunostained with rabbit anti-βIII tubulin antibody (1:500) to visualize the neuronal cytoskeleton (gray) and with a mouse anti-puromycin antibody (1:500) to analyze newly synthesized proteins (heatmaps). Secondary anti-rabbit DyLight 405 (1:200, 611-146-002). Scale bar, 50 μm. Fig 2. PMID: 32581689.

NanoLoc-mediated control of GFP subcellular localization. Representative confocal microscopy images of HEK293/GFP#1 cells transiently transfected for the expression of VHHLoc？variants and analyzed 72？h post-transfection. Subcellular compartment (red, column 1); GFP (green, column 2); VHH (blue, anti-HA, column 3); colocalization of subcellular compartment and VHH shown in merged images (purple, column 4); colocalization of GFP and VHH shown in merged images (cyan, column 5); colocalization of subcellular compartment and GFP shown in merged images (yellow, column 6). Scale bars: 5？μm. Brightness and contrast levels were adjusted and images of cells treated the same were subjected to the same adjustment. Pseudo-coloring was applied to the subcellular compartment stain and VHH images for the plasma membrane and the nucleus. Primary antibodies: rabbit anti-HA (VHH), 1:250, mouse anti-calnexin (endoplasmic reticulum membrane) 1:50; mouse anti-PMP70 (peroxisome) 1:50, with secondary antibodies: goat anti-rabbit DyLight 405 conjugated, (p/n 611-146-002) 1:200, goat anti-rabbit DyLight 549 conjugated, (p/n 611-142-002) 1:500, and goat anti-mouse DyLight 549 conjugated, (1:500). Fig 1. PMID: 33763602.

DyLight？ 405 Fluorescence absorption

Properties of DyLight？ Fluorescent Dyes.