Anti IgG (H&L)   DyLight (商標名)

Properties of DyLightTM Fluorescent Dyes.

DyLightTM dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. DyLightTM dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods. This image shows anti-histone detection using a DyLightTM 488 conjugate (green). Anti-Tubulin was detected using a DyLightTM 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).

DyLight 488？ Goat anti-Rabbit IgG used in confocal microscopy shows detection of changes in AKTpS473 localization in EGF treated A431 cells. A Leica TCS SP5 was used to detect tubulin (cyan) stained with Rockland's DyLight 488？ Goat anti-Rabbit IgG, and AKT (red) stained with Rockland's MAb anti-AKT pS473 p/n 200-301-268. The images show a weak diffuse staining of AKT in serum starved resting cells (''No treatment''), and a marked activation and migration of AKT to the periphery of the cells upon stimulation with the mitogen EGF (''+ EGF 15 min'').

Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (p/n 611-1102). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (p/n 611-1102) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.

DyLightTM 488 Fluorescence Spectra.