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Synaptophysin density alterations in phenylalanine-treated hippocampal neurons. Hippocampal “sandwich” co-cultures were treated with Phe/Doxy for 72？h. Cells were double-stained with NF200 (red) and synaptophysin (green); nuclei were marked by Hoechst 33258 (blue). The number of synaptophysin-positive spots was normalized to the total volume of neurofilaments (NF200 expression) after three-dimensional reconstruction of the marker signals (representative images in (B). At least ten fields (1200x) for each condition from three independent experiments were analyzed. (A) The density of synaptophysin-positive spots was significantly increased by 20？mM Phe (*p？<？0.05 versus control; One-way ANOVA and Dunnett’s post-test). Treatment with 25？μM Doxy showed a trend towards a reduction in synaptophysin density, increasing towards control levels. Figure 11. PMID: 26510963.
Phenylalanine-induced dendritic sprouting alterations in hippocampal neurons prevented by doxycycline co-treatment. Hippocampal “sandwich” co-cultures were treated with Phe/Doxy for 72？h. The neuron fractions were stained with NF200 and nuclei were marked by Hoechst 33258. At least eight fields (600x) for each condition from three independent experiments were analyzed. The number of dendritic branches (A) and Sholl intersections (B) was significantly decreased by Phe treatments. Treatment with 25？μM Doxy significantly counteracted the dendritic alterations induced by 20？mM Phe. *p？<？0.05, **p？<？0.01, ***p？<？0.001 versus control. °p？<？0.05？vs 20？mM Phe. One-way ANOVA and Tukey’s post-test. Figure 10. PMID: 26510963.
Properties of DyLight？ Fluorescent Dyes.
DyLight？ dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. DyLight？ dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods. This image shows anti-histone detection using a DyLight？ 488 conjugate (green). Anti-Tubulin was detected using a DyLight？ 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).
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Synaptophysin density alterations in phenylalanine-treated hippocampal neurons. Hippocampal “sandwich” co-cultures were treated with Phe/Doxy for 72？h. Cells were double-stained with NF200 (red) and synaptophysin (green); nuclei were marked by Hoechst 33258 (blue). The number of synaptophysin-positive spots was normalized to the total volume of neurofilaments (NF200 expression) after three-dimensional reconstruction of the marker signals (representative images in (B). At least ten fields (1200x) for each condition from three independent experiments were analyzed. (A) The density of synaptophysin-positive spots was significantly increased by 20？mM Phe (*p？<？0.05 versus control; One-way ANOVA and Dunnett’s post-test). Treatment with 25？μM Doxy showed a trend towards a reduction in synaptophysin density, increasing towards control levels. Figure 11. PMID: 26510963.
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| 別品名 |
Donkey anti-Mouse IgG DyLight 488TM Conjugated Antibody, Donkey anti Mouse IgG Antibody DyLight 488TM Conjugation
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| 交差種 |
Mouse
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| 免疫動物 |
Donkey
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| 標識物 |
DyLightTM 488
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| 精製度 |
Affinity Purified
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| 参考文献 |
[Pub Med ID]26510963
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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610-741-002
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 法規制 |
毒
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| 保存温度 |
４℃
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