Anti IgG (H&L)  
DyLight (商標名)

Skin cell cultures from patients with AD-HIES display TNF-α？sensitive defects in wound healing.(E) Western blot of nuclear and cytoplasmic fractions from KCs obtained from HVs and AD-HIES patients (KCs were cultured with TNF-α or diluent). Figure 3. PMID: 30035749.

TGF‐β‐mediated crosstalk between pericytes and CRC cells modulates pericyte secretome. (A) Incubation in HCT116 CM for 1？h induces SMAD3 phosphorylation in PC, as assessed by western blot. Exogenous recombinant TGF‐β (10？ng・mL−1) was used as a positive control, and β‐actin was used as loading control (n？=？3).？ Fig. 5. PMID: 32767843.

Insulin‐like growth factor‐binding protein 3 increases CRC cell migration and invasion through Akt activation. (D) Treatment with 50？ng・mL−1？IGFBP‐3 for 72？h promotes the expression of N‐cadherin in HCT116 cells as assessed by western blot (top panel). Phosphorylation status of Akt (middle panel) and MAPK (bottom panel) in HCT116 cells treated with 50？ng・mL−1？IGFBP‐3 for 15？min. Representative images of three independent experiments (n？=？3). Numbers indicate the expression fold change relative to the loading control. Fig. 7. PMID: 32767843.

DyLight？ dyes can be used for two-color western blot detection with low background and high signal. Anti-tubulin was detected using a DyLight？ 680 conjugate. Anti-TNFa was detected using a DyLight？ 800 conjugate. The image was captured using the OdysseyR Infrared Imaging System developed by LI-COR.

DyLight？ 680 Fluorescence Spectra.

Properties of DyLight？ Fluorescent Dyes.