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Differentiated rMSCs immunofluorescence. Negative control (A). Cells were stained with an antibody against b-III-tubulin (green) after 14 days (B) and 28 days (C) of differentiation using Rabbit Anti-Mouse IgG DyLight？488 (p/n 610-441-002). After 28 days of differentiation rMSCs were positive for Gad67 (green) (D); choline acetyltransferase (Acht) (red) (E, F) and tyrosine hydroxylase (Th) (green) (G, H). Cells positive for Gad67 (green) also co-expressed Cxcr4 (yellow) (I, J); and cells positive for Th (green) co-expressed Cxcr4 (yellow) as well (K, L). In each experiment the nuclei were counterstained with 40 ,6-diamidino-2-phenylindole (DAPI) (blue). In our study only cells which were positive stained and had the shape characteristic for neuronal cells, were qualified as positive for examined markers (white arrows). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) Fig. 5. PMID: 25712637.
Differentiated rMSCs immunofluorescence. Cells were stained with an antibody against b-III-tubulin (green) using Rabbit Anti-Mouse IgG DyLight？488 (p/n 610-441-002). Differences in the number of primary dendrites and branching dendrites using various differentiation schemes. Negative control (A); control, differentiation using a basic differentiation medium (B and C); long-term imipramine treatment (D); CACM treatment (E); CACM + desipramine (F and G); CACM + fluoxetine (H, I). In each experiment, the nuclei were counterstained with 40 ,6- diamidino-2-phenylindole (DAPI) (blue). In our study, only cells that were positive stained and had the shape that is characteristic for neuronal cells were qualified as positive. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) Fig. 4. .PMID: 25712637.
(B) Flow cytometric detection of the cell-surface interleukin-4 receptor on cloned human GBM8401-luc cells. Fig 1. PMID: 22393293.
DyLight？ dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. DyLight？ dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods. This image shows anti-histone detection using a DyLight？ 488 conjugate (green). Anti-Tubulin was detected using a DyLight？ 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).
DyLight？ 488 Fluorescence Spectra.
Properties of DyLight？ Fluorescent Dyes.
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Differentiated rMSCs immunofluorescence. Negative control (A). Cells were stained with an antibody against b-III-tubulin (green) after 14 days (B) and 28 days (C) of differentiation using Rabbit Anti-Mouse IgG DyLight？488 (p/n 610-441-002). After 28 days of differentiation rMSCs were positive for Gad67 (green) (D); choline acetyltransferase (Acht) (red) (E, F) and tyrosine hydroxylase (Th) (green) (G, H). Cells positive for Gad67 (green) also co-expressed Cxcr4 (yellow) (I, J); and cells positive for Th (green) co-expressed Cxcr4 (yellow) as well (K, L). In each experiment the nuclei were counterstained with 40 ,6-diamidino-2-phenylindole (DAPI) (blue). In our study only cells which were positive stained and had the shape characteristic for neuronal cells, were qualified as positive for examined markers (white arrows). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) Fig. 5. PMID: 25712637.
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| 別品名 |
rabbit anti-Mouse IgG Antibody DyLightTM 488 conjugation, rabbit anti-Mouse IgG DyLightTM488 conjugated Antibody
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| 交差種 |
Mouse
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| 免疫動物 |
Rabbit
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| 標識物 |
DyLightTM 488
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| 精製度 |
Affinity Purified
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| 参考文献 |
[Pub Med ID]25712637
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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610-441-002
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 法規制 |
毒
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| 保存温度 |
４℃
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