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Comparison between EXTL2 + / + and EXTL2 − / − mice with regard to the expression and localization of GAGs and SDCs Liver sections (Vibratome, 30 μm/slice) before administration of CCl4 were stained with various antibodies against GAGs. HepSS-1 (a and b), NAH46 (c and d) and JM403 (e and f) recognize HS structures, -[GlcUAβ1-4GlcNSα1-4]n-, -[GlcUAβ1-4GlcNAcα1-4]n- and -[GlcUAβ1-4GlcNH2α1-4]n- respectively. F58-3G10 (g and h) recognizes HS neo-epitope generated by digesting HS with heparitinese. 2-B-6 (iand j) recognizes 4-O-sulfated CS neo-epitope generated by digesting CS with CSase ABC; CS-56 (k and l) recognizes CS structures, -[GlcUAβ1-3GalNAc(6-O-sulfate)β1-4]n-; SDC2 (m and n), SDC4 (o and p). The following secondary antibodies were used to detect primary antibodies: Alexa FluorR 594 donkey anti-rabbit IgG (H + L) [1:400], goat anti-mouse IgM (μ chain) antibody DyLight? 488 conjugated [1:400] (p/n 610-141-007), and AffiniPure Fab Fragment goat anti-mouse IgG (H + L) DyLight? 488 conjugated [1:250]. Figure 4. PMID: 23734945.
DyLight? dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image.? DyLight? dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods.? This image shows anti-histone detection using a DyLight? 488 conjugate (green).? Anti-tubulin was detected using a DyLight? 549 conjugate (red). ?Nuclei were counter-stained using DAPI (blue).? The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).
Properties of DyLight? Fluorescent Dyes.
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Comparison between EXTL2 + / + and EXTL2 − / − mice with regard to the expression and localization of GAGs and SDCs Liver sections (Vibratome, 30 μm/slice) before administration of CCl4 were stained with various antibodies against GAGs. HepSS-1 (a and b), NAH46 (c and d) and JM403 (e and f) recognize HS structures, -[GlcUAβ1-4GlcNSα1-4]n-, -[GlcUAβ1-4GlcNAcα1-4]n- and -[GlcUAβ1-4GlcNH2α1-4]n- respectively. F58-3G10 (g and h) recognizes HS neo-epitope generated by digesting HS with heparitinese. 2-B-6 (iand j) recognizes 4-O-sulfated CS neo-epitope generated by digesting CS with CSase ABC; CS-56 (k and l) recognizes CS structures, -[GlcUAβ1-3GalNAc(6-O-sulfate)β1-4]n-; SDC2 (m and n), SDC4 (o and p). The following secondary antibodies were used to detect primary antibodies: Alexa FluorR 594 donkey anti-rabbit IgG (H + L) [1:400], goat anti-mouse IgM (μ chain) antibody DyLight? 488 conjugated [1:400] (p/n 610-141-007), and AffiniPure Fab Fragment goat anti-mouse IgG (H + L) DyLight? 488 conjugated [1:250]. Figure 4. PMID: 23734945.
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| 別品名 |
Goat Anti-Mouse IgM (mu chain) Antibody DyLight 488TM Conjugated, Goat Anti Mouse IgM mu chain DyLight 488TM Conjugated Antibody
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| 交差種 |
Mouse
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| 免疫動物 |
Goat
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| 標識物 |
DyLightTM 488
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| 精製度 |
Affinity Purified
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| 参考文献 |
[Pub Med ID]23734945
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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610-141-007
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 法規制 |
毒
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| 保存温度 |
4℃
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