Anti IgM (μ chain)  
DyLight (商標名)

Western Blot analysis of IgG and IgM antibodies against？R. helvetica？whole cell antigen demonstrates the lipopolysaccaride (LPS) ladders and specific reactions against？R. helvetica？proteins in the 110？150-kDa region in serum for IgG for patients 1, 32 and 33 and for IgM for patients 3, 8, 10, 17, 20 and 22 in dilution 1:200. Lane P(h) demonstrates specific proteins and the LPS ladders reacting with a positive human serum and P(r) with a polyclonal rabbit antiserum. N(h) represent a negative human serum control. Secondary antibody Anti-human IgG DyLight？549 (p/n 609？142-123 ) and Anti-human IgM DyLight？ 549 (p/n 609？142-007). Figure 1. PMID: 34712390.

Identification of four immunogenic MERS-CoV-S epitopes. (a)？Schematic representation of the MERS-CoV-S protein. The N-terminal domain (NTD), receptor-binding domain (RBD), S1/S2 cleavage site (S1/S2), fusion peptide (FP), S2‘ cleavage site (S2‘), heptad repeat 1 and 2 (HR1, HR2), transmembrane domain (TM) and cytoplasmic domain (CD) are illustrated.？(b)？Microarray of 15-mer peptides spanning the complete MERS-CoV-S protein with a 13 amino acid (AA) overlap. Immunogenic B-cell peptides are marked with red lines.？(c？f)？IgG binding to the respective peptides on MERS-CoV-S was measured in fluorescence intensity (as arbitrary fluorescence units, AFU), depicted as transformed values (areas sinus hyperbolicus (asinh), y axis), in all booster study participants (n？=？10). Mean levels of peptide-binding IgG at baseline (Day (D) 0 (D0), white boxes) and 28 days after booster vaccination (Boost Day (B:D) 28 (B:D28), gray boxes) were compared using a two-sided Wilcoxon matched-pairs signed rank test and are depicted for each peptide (x-axis) within the immunogenic epitopes？c？AA 535-553 (AA 535？549,？p？=？0.014; AA 537？551,？p？=？0.002; AA 539？553,？p？=？0.002),？d？AA 887？913 (AA 887？901,？p？=？0.027; AA 889？903,？p？=？0.006; AA 891？905,？p？=？0.002; AA 897？911,？p？=？0.002; AA 899？913,？p？=？0.002),？e？AA 1225？1247 (AA 1225？1239,？p？=？0.002; AA 1227？1241,？p？=？0.002; AA 1229？1243,？p？=？0.002; AA 1231？1245,？p？=？0.002; AA 1233？1247,？p？=？0.004) and？f？AA 1333？1353 (AA 1333？1347,？p？=？0.002; AA 1335？1349,？p？=？0.002; AA 1337？1351,？p？=？0.002; AA 1339？1353,？p？=？0.002). Boxes indicate 25？75 percentile; whiskers are min. to max.; medians are shown as horizonal lines within the boxes. *p？<？0.05, **p？<？0.005. Source data are provided as a Source Data file. Fig. 5. PMID: 35853863.

Properties of DyLight？ Conjugates.

DyLight？ dyes can be used for two-color Western Blot detection with low background and high signal.？ Anti-tubulin was detected using a DyLight？ 549 conjugate.？ Anti-TNFa was detected using a DyLight？ 649 conjugate. The image was captured using the Typhoon？ 9410 Imaging System.