Anti Biotin  

ELISA Results of purified Rabbit Anti-Biotin Antibody tested against BSA-Biotin of immunogen. Each well was coated in duplicate with 1.0μg of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Goat anti-Rabbit IgG HRP conjugated (p/n 611-103-122), and TMB substrate (p/n TMBE-1000).

Localization of Pro-ChoPLs during drug-induced autophagy. Imaging of Pro-ChoPLs in 20？μM PI-103 (24？h and 6？h), and in 75 mM DCA (24？h)-treated HCT116？BAX-ko cells by immuno-electron microscopy. Pro-Cho was added together with the vehicle- or PI-103 treatment, but in the last 6？h of DCA treatment to adjust for the difference in the timing of autophagy onset between the two treatments (Figure S2). No Pro-Cho was added for the negative, vehicle-treated control. The cells were fixed, sectioned and Pro-ChoPLs reacted with biotin-azide. The sections were incubated with anti-biotin antibodies (p/n 600-401-098) and protein A gold, counterstained with uranyl acetate and imaged by transmission electron microscopy. Arrows indicate various cellular structures: AV, autophagic vacuole; ER, endoplasmic reticulum; ICS, intercellular space; Mt, mitochondrion; NE, nuclear envelope; Nu, nucleus; PM, plasma membrane; Ve, vesicle. Fig 3. PMID:？31517566

Colocalization of ceroids and LAMP-2 (lysosomal marker) in the Mox50 model (representative images obtained from one donor, n = 3, with a Leica TCS SP5 microscope). Rabbit anti-biotin (p/n 600-401-098) antibody was used to directly detect biotin in the SenTraGor^(R)？reagent. Fig 8. PMID:？39337629