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Cell lysates of Jurkat (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 tau (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : Recombinant Human YWHAZ
Lane 2. : Recombinant Human YWHAB
Lane 3. : Recombinant Human YWHAE
Lane 4. : Recombinant Human YWHAH
Lane 5. : Recombinant Human YWHAG
Lane 6. : Recombinant Human SFN
Lane 7. : Recombinant Human YWHAQ
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : MDA-MB-21 cell lysate
Lane 2. : HeLa cell lysate
Lane 3. : A431 cell lysate
Lane 4. : NIH3T3 cell lysate
Lane 5. : 293T cell lysate
Lane 6. : HepG2 cell lysate
ICC/IF analysis of 14-3-3 tau in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 tau antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
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| 別品名 |
tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein theta polypeptide, YWHAQ, 1C5, HS1, 14-3-3 tau, Tyrosine 3/tryptophan 5 -monooxygenase activation protein, theta polypeptide, tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, theta polypeptide 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14 3 3 tau, 14 3 3 theta, KCIP1, Protein HS1, Protein kinase C inhibitor protein 1, tyr3/trp5 monooxygenase activation protein, theta, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, theta polypeptide.
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| 種由来 |
Human
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immuno Fluorescence
Immunocytochemistry (cell)
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| 免疫動物 |
Mouse
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| クローン |
AT1A1
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| 抗体クラス |
IgG1κ
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| 抗原部位 |
a.a.1-245
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein G
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| Accession No.(Gene/Protein) |
NP_006817, P27348
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| 概要 |
The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau andtau that have been identified in mammals. The 14-3-3 tau, a subtype of the 14-3-3 family of proteins, was found in T Cells, brain and testes. This 14-3-3 tau is upregulated in patients with amyotrophic lateral sclerosis.
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| 参考文献 |
Liu YC, et al., (1996) J Biol Chem 271:14591-14595
Xiao B, et al., (1995) Nature 376:188-191
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| メーカー |
品番 |
包装 |
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ATG
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ATGA0132
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100 UL
[1mg/ml]
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-７０℃
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