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※サムネイル画像をクリックすると拡大画像が表示されます。
The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IL-33 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: Jurkat cell lysate
Flow cytometry analysis of IL-33 in Jurkat cells. The cell was stained with AIL0824 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
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The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IL-33 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: Jurkat cell lysate
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| 別品名 |
Interleukin 33, DVS27, NF-HEV, NKHEV, C9orf26, IL-33, Interleukin 33 CHROMOSOME 9 OPEN READING FRAME 26, DKFZp586H0523, DVS27 related protein, IL 1F11, IL 33, IL1F11, Interleukin 1 family member 11, INTERLEuKIN 33 NFHEV, Interleukin 33 precursor, Interleukin33, NF HEV, NFHEV, Nuclear factor from high endothelial venules, RP11 575C20.2, il33
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| 種由来 |
Human
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immuno Fluorescence
Immunocytochemistry (cell)
Flow Cytometry
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| 免疫動物 |
Mouse
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| クローン |
4E9
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| 抗体クラス |
IgG2bκ
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| 抗原部位 |
a.a.112-270
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein G
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| Accession No.(Gene/Protein) |
NP_254274, O95760
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| 概要 |
Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.
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| 参考文献 |
Brint Ek, et al., (2005) J Biol Chem. 20:277(51).
Schmitz, et al., (2005) immunity. 23:479.
Baekkevold ES, et al., (2003) Am.J.Pathol. 163(1):69-90.
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| メーカー |
品番 |
包装 |
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ATG
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AIL0824
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50 UL
[1mg/ml]
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-７０℃
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