PI3-Kinase Activity ELISA: Pico

※サムネイル画像をクリックすると拡大画像が表示されます。

使用目的	PI-3 kinase活性測定キットです。Kinase反応の後のサンプルを競合ELISA法によりPI(3,4,5)P₃を測定することで、活性測定が可能です。
概要	The PI3-Kinase Activity ELISA: Pico is a competitive ELISA that measures Class I Phosphoinositide 3-kinases (PI3-K) activity directly through its reaction product PtdIns(3 4 5)P3 (PIP3). This assay can be used for in vitro determination of PI3-K activity in cancer tissues and cell lines or compound screening for drug discoveryAssay sensitivity: This assay can measure as low as 0.22 pmol PIP3 and detect activity from as little as 31 ng/mL PI3-K enzymeAssay Incubation time: 2.5 hrSample Type: Purified and/ or Immunoprecipitated (IP) PI3-K Enzyme (see IP protocol at the end of the K-1000s TDS)PI3-K reactions are run with the substrate PI(4 5)P2 (PIP2). The enzyme reactions PIP3 standards and controls are then mixed and incubated with PIP3 binding protein that is highly specific and sensitive to PIP3. This mixture is then transferred to a PIP3-coated microplate for competitive binding. Afterwards a peroxidase-linked secondary detector and colorimetric detection is used to detect the amount of PIP3 produced by PI3-K. The amount of enzyme product (PIP3) produced is determined by comparing the enzyme reactions with a PIP3 standard curve.PI3-K's are ubiquitously expressed lipid kinases which phosphorylate PIP2 at the 3'-hydroxyl of the inositol ring producing PIP3. PIP3 has shown to be an important lipid second messenger with key roles in fundamental cellular responses such as proliferation and survival. Due to these interesting biological roles PI3-K and PIP3 have become attractive targets for research and drug development in many diseases including inflammation and cancer.Echelon has multiple formats available for Class III PI3-K. Please see this PI3-K Assay Comparison Guide. Powered by Bioz See more details on BiozNotice to Purchaser: Echelon Biosciences products are sold for research and development purposes only and are not to be incorporated into products for resale without written permission from Echelon Biosciences. This kit and all non-radioactive competitive assays for determining phosphoinositide-3-kinase (PI3-K) activity is covered by Echelon Biosciences Inc. U.S. Patent 7 067 269. The purchase of this product includes a limited non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. For inquiries email busdev@echelon-inc.com.Product Keywords: PI3-Kinase PI3K Phosphatidylinositol 3-kinases PI 3-kinases PI3Ks K-1000
構成内容	・#K-1001s, PI(3,4,5)P₃ Coated Strip-well Detection Plate, 1 plate ・#K-1008, PI(4,5)P₂ Substrate, diC₈, 23 nmol ・#K-1003s, PI(3,4,5)P₃ Standard, diC₈, 0.9 nmol ・#K-1006, PI(3,4,5)P₃ Detector, 20 μL ・#K-1005s, Detection Buffer, 10 mL ・#K-SEC1, Secondary Detector, 300 μL ・#K-TBST, 10x TBS-T Buffer, 20 mL ・#K-TMB1, TMB Solution, 12 mL ・#K-STOPt, 1 N H₂SO₄ Stop Solution, 10 mL ・#K-KBZ, 5x KBZ Kinase Reaction Buffer, 4 mL ・#K-EDTA, 100 mM EDTA Solution, 500 μL ・#Incubation Plate, Colored 96-well polypropylene U-bottom plate, 1 plate ・#Plate Sealers, Clear acetate sheet, 1 side adhesive, 3 selas

メーカー	品番	包装
ECL	K-1000S	1 KIT [96-well]

希望販売価格	¥111,000

※表示価格について

当社在庫	なし
納期目安	1週間程度
法規制	安・化管
保存温度	４℃

メーカー情報
メーカー名	Echelon Biosciences Inc.
略号	ECL

関連情報
同一品名検索

※当社では商品情報の適切な管理に努めておりますが、表示される法規制情報は最新でない可能性があります。
　また法規制情報の表示が無いものは、必ずしも法規制に非該当であることを示すものではありません。
　商品のお届け前に最新の製品法規制情報をお求めの際はこちらへお問い合わせください。　

※当社取り扱いの試薬・機器製品および受託サービス・創薬支援サービス（納品物、解析データ等）は、研究用としてのみ販売しております。
　人や動物の医療用・臨床診断用・食品用としては、使用しないように、十分ご注意ください。
　法規制欄に体外診断用医薬品と記載のものは除きます。

※リンク先での文献等のダウンロードに際しましては、掲載元の規約遵守をお願いします。