別品名 |
GST, Glutathione-S-Transferase
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標識物 |
DyLightTM 680
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay
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免疫動物 |
Mouse
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抗体クラス |
IgG1κ
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クローン |
3D4
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Tag情報 |
GST
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形状 |
凍結乾燥品
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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Properties of DyLightTM Conjugates.
Rockland Mouse-a-GST (200-301-200 lot 24882, blue), Rabbit anti-Transferrin (109-4134 lot 3033), and Goat-anti-Alpha-1-Anti-Trypsin (100-101-147 lot 5842) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (611-742-127 lot 21100, shown as green) ? DyLight 488 Donkey anti-Mouse IgG (610-741-124 lot 21095, shown as blue), ?and DyLight 649 Donkey anti-Goat IgG (605-743-125 lot 20834, shown as red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the BioRad VersaDoc System.
DyLightTM dyes can be used for two-color western blot detection with low background and high signal. Anti-tubulin was detected using a DyLightTM 680 conjugate. Anti-TNF? was detected using a DyLightTM 800 conjugate. The image was captured using the OdysseyR Infrared Imaging System developed by LI-COR.
Properties of DyLight? Conjugates.
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Properties of DyLightTM Conjugates.
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メーカー |
品番 |
包装 |
RKL
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200-344-200
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100 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
4℃
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