別品名 |
GST, Glutathione-S-Transferase
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標識物 |
DyLightTM 649
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精製度 |
Affinity Purified
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免疫動物 |
Mouse
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抗体クラス |
IgG1κ
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クローン |
3D4
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Tag情報 |
GST
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形状 |
凍結乾燥品
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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Rockland Mouse a GST (200 301 200 lot 24882, blue), Rabbit anti Transferrin (109 4134 lot 3033), and Goat anti Alpha 1 Anti Trypsin (100 101 147 lot 5842) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS PAGE, transferred to 0.2 um PVDF using the BioRad Trans Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB 070 over night at 4C). Detection shown was using DyLight549 Donkey anti Rabbit IgG (611 742 127 lot 21100, shown as green) ? DyLight 488 Donkey anti Mouse IgG (610 741 124 lot 21095, shown as blue), ?and DyLight 649 Donkey anti Goat IgG (605 743 125 lot 20834, shown as red) at 1:10000 (in MB 070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the BioRad VersaDoc System.
Properties of DyLightTM Conjugates.
DyLightTM dyes can be used for two-color western blot detection with low background and high signal.? Anti-tubulin was detected using a DyLightTM 549 conjugate.? Anti-TNFa was detected using a DyLightTM 649 conjugate. The image was captured using the TyphoonTM 9410 Imaging System.
Rockland Mouse-a-GST (200-301-200 lot 24882, blue), Rabbit anti-Transferrin (109-4134 lot 3033), and Goat-anti-Alpha-1-Anti-Trypsin (100-101-147 lot 5842) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4°C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (611-742-127 lot 21100, shown as green) ? DyLight 488 Donkey anti-Mouse IgG (610-741-124 lot 21095, shown as blue), ?and DyLight 649 Donkey anti-Goat IgG (605-743-125 lot 20834, shown as red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the BioRad VersaDoc System.
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Rockland Mouse a GST (200 301 200 lot 24882, blue), Rabbit anti Transferrin (109 4134 lot 3033), and Goat anti Alpha 1 Anti Trypsin (100 101 147 lot 5842) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS PAGE, transferred to 0.2 um PVDF using the BioRad Trans Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB 070 over night at 4C). Detection shown was using DyLight549 Donkey anti Rabbit IgG (611 742 127 lot 21100, shown as green) ? DyLight 488 Donkey anti Mouse IgG (610 741 124 lot 21095, shown as blue), ?and DyLight 649 Donkey anti Goat IgG (605 743 125 lot 20834, shown as red) at 1:10000 (in MB 070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the BioRad VersaDoc System.
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メーカー |
品番 |
包装 |
RKL
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200-343-200
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100 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
4℃
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