Anti IgG (H&L)  
DyLight (商標名)

FYF-ITK mutant nucleofected cells display defective PLCγ1 phosphorylation. (A) Thymocytes isolated from ITK−/−？mice and nucleofected with cDNA constructs encoding GFP-tagged WT-, FYF-, or K390R-ITK fusion proteins, or mock-nucleofected were stimulated or not with anti-mouse CD3ε antibodies and then analyzed by flow cytometry using Alexa 647-conjugated anti-PLCγ1 pY783 antibodies as described in the？Materials and Methods？section. Results are displayed as cell number versus fluorescence intensity. In each panel the grey dotted line histograms represent mock-nucleofected cells that were not stimulated as negative controls for setting an electronic gate (vertical line) for calculation of positive cells. Histograms of non-stimulated, nucleofected or mock-nucleofected cells were similar. The black dotted line histograms represent K390R-ITK nucleofected cells that were stimulated. The solid black line histograms represent WT-ITK nucleofected (left panel) and FYF-ITK mutant nucleofected (right panel) cells that were stimulated. The table inset lists anti-PLCγ1 MFI of the displayed stimulated cell histograms.？Figure 2. PMID: 23028816.