Anti IgG (H&L)  
DyLight (商標名)

Colocalization of p57kip2 with oligodendroglial binding proteins.？？Using primary antibodies: rabbit anti-p57kip2 [1/500], rabbit anti-LIMK-1 (1/500), mouse anti-CDK2 (1/75) and mouse anti-Mash1 (1/200), secondary antibodies Alexa Fluors and DyLight 405-conjugated antibodies (p/n# 603-146-126), and nuclei were stained with (DAPI).？(D？I′′′) Representative images illustrating nuclear and cytoplasmic p57kip2 localization within oligodendroglial cells after 6 d under differentiation conditions. Colocalization with both LIMK-1 (D？D′′′,E？E′′′) and CDK2 (F？F′′′,G？G′′′) was observed, whereas only nuclear coexpression of Mash1 and p57kip2 in immature oligodendroglial cells (H？H′′′,？I？I′′′) was found. Scale bars, 20 μm. Fig5. PMID: 25609610.

p73 protein induction in response to teriflunomide stimulation.？Primary antibodies mouse anti-MBP (1/250) and rabbit anti-p73 (1/100), secondary antibodies Alexa Fluors and DyLight 405-conjugated antibody (p/n 603-146-126), and nuclei were stained with (DAPI).？ a？c”’？Double immunostaining and its quantification confirmed that early teriflunomide pulses (a 24？h pulse stimulation [scheme II[ with teriflunomide followed by a 48-hr) result in cells displaying strong TAp73 signals (arrowheads in？b) correlating also with MBP positivity (dashed bars in？a). Late teriflunomide pulses (a short-term pulse at a later time point from 48 to 72？h [scheme III]？) could not boost both protein markers. Scale bars, 20？μm.？d？g？Western blot analysis confirmed that early short-term teriflunomide pulses (a 24？h pulse stimulation [scheme II[ result in a strong induction of CNPase. Fig. 4. PMID: 29534752.

Properties of DyLight？ Fluorescent Dyes.