Anti HA Epitope Tag  
DyLight (商標名)

Immunofluorescence of Anti-HA Epitope Tag Antibody DyLight？405 Conjugated.？(E)？representative confocal images of immunostaining of a SH-SY5Y cell expressing both Myc-PINK1 (red) and HA-CHIP (blue). Mitochondria were stained with TOM20 (green).？(F)？SH-SY5Y cells were treated for 6 h with 10 μm？MG132 before fixation. Representative confocal images of immunostaining using endogenous PINK1 (red) and endogenous CHIP (green). Nuclei were counterstained with DAPI (blue).？Scale bars,？10 μm. Figure 1. PMID: 29242192.

Immunofluorescence double staining using Anti-HA Epitope Tag Antibody DyLight？405 Conjugated.Intracellular distribution of tagged OGR1 visualized by sortase A-mediated pulse labeling and immunostaining. (A) Fluorescent images of the tagged OGR1 enzymatically labeled with an Alexa Fluor 488-modified peptide (green) and (B) immunostained with a DyLight 405-conjugated anti-HA-antibody (blue), obtained with a confocal microscope at the mildly acidic extracellular pH 6.6. The original fluorescence intensities of the labeled and immunostained cells were subtracted with the mean intensities of the green and blue autofluorescence of the intact cells, respectively. The yellow lines represent the outlines of the whole cells and the intracellular regions. Scale bar: 10 mm. (C) Distribution ratio of the labeled and immunostained OGR1 between the cell surface and the intracellular compartments obtained by image analysis of panels A and B. The color bars represent the mean ratio from four cells, and the error bars represent the S.D. FIG. 3. PMID: 29655915.

Properties of DyLight？ Fluorescent Dyes.