Anti HA Epitope Tag  
DyLight (商標名)

Immunofluorescence using Anti-HA Epitope Tag Antibody DyLight？800 Conjugated. Tirzepatide (TZP) differentially induces internalization of the GIP and GLP-1 receptors.(E？H) Studies using receptors containing an N-terminal HA-epitope tag and a C-terminal EGFP fusion are presented. (E) Changes in surface GIPR 60 minutes after treatment with ligand were measured by anti-HA immunofluorescence. Data are normalized to 1 μM GIP(1-42) values. For GIPR, tirzepatide induced internalization with an EC50？(SEM,？n) of 18.1 nM (5.7, 4), while GIP(1-42) displayed a potency of 18.2 nM (9.7, 4). (G) Representative confocal images of HA-GIPR-EGFP cells detecting EGFP fluorescence following treatment with vehicle, 100 nM GIP(1-42), or 100 nM tirzepatide. Figure 2. PMID: 32730231.

Immunofluorescence using Anti-HA Epitope Tag Antibody DyLight？800 Conjugated. Tirzepatide (TZP) differentially induces internalization of the GIP and GLP-1 receptors.(E？H) Studies using receptors containing an N-terminal HA-epitope tag and a C-terminal EGFP fusion are presented. (F) Changes in surface GLP-1R 30 minutes after treatment with ligand detected by anti-HA immunofluorescence. Data are normalized to 1 μM GLP-1(7-36) values. For GLP-1R, tirzepatide was partially efficacious at 43.6% (7.9, 3) with an EC50？of 101.9 nM (29.8, 3), while GLP-1(7-36) showed a potency of 22.2 nM (1.86, 3). (H) Representative confocal images of HA？GLP-1R？EGFP cells detecting EGFP fluorescence following treatment with vehicle, 100 nM GLP-1(7-36), or 100 nM tirzepatide. Scale bars: 20 μm. Figure 2. PMID: 32730231.

DyLight？ dyes can be used for two-color Western Blot detection with low background and high signal.？ Anti-tubulin was detected using a DyLight？ 680 conjugate.？ Anti-TNFa was detected using a DyLight？ 800 conjugate. The image was captured using the OdysseyR Infrared Imaging System developed by LI-COR.

Properties of DyLight？ Conjugates.