| 別品名 |
GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.
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| 標識物 |
DyLightTM 488
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Dot Blot
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| 免疫動物 |
Goat
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| 非交差(吸収処理)種 |
Human
Mouse
Rat
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| Accession No.(Gene/Protein) |
P42212
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| Tag情報 |
GFP
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| 形状 |
凍結乾燥品
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| 参考文献 |
[DOI]10.1101/2021.11.21.469249
[Pub Med ID]29288597, 29709033, 30811988, 31201094, 24398145, 21527921, 24997270, 27689803, 28620067, 29564795, 29662169, 31130512, 33603067, 35645783, 27599206, 35343899, 33851141, 34083536, 34592156
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Bot of Goat Anti GFP (GOAT) Antibody DyLightTM 488 Conjugated. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB 210 0500). Lane 2: GFP / HEK293T Whole Cell Lysate Reduced [0.05/10ug]. Lane 3: HEK293T Whole Cell Lysate Reduced (p/n W09 001 GX5) [10ug]. Lane 4: GFP / NIH 3T3 Whole Cell Lysate Reduced [0.05/10ug]. Lane 5: NIH 3T3 Whole Cell Lysate Reduced (p/n W10 000 358) [10ug]. Lane 6: GFP / PC 12 Whole Cell Lysate Reduced [0.05/10ug].Lane 7: PC 12 Whole Cell Lysate Reduced (p/n W12 001 GL9) [10ug]. Secondary Antibody: Goat Anti GFP (GOAT) Antibody DyLightTM 488 Conjugated at 1.0ug/mL overnight at 2 8C. Blocking Buffer: Blocking Buffer for Fluorescent Western Blotting (p/n MB 070) for 1 hour at RT. Expect: 27kDa.
Multi-color Immunofluorescence Microscopy using DyLightTM dyes. Tissue: human breast carcinoma. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Anti-Histone and Anti-Tubulin antibody at 10 ug/mL for 1 h at RT. Secondary antibody: DyLightTM 488 conjugate and DyLightTM 549 conjugate goat secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone is nuclear and Tubulin is cytoplasmic. Staining: Anti-Histone detection using a DyLightTM 488 conjugate (green) fluorescent signal and Anti-Tubulin was detected using a DyLightTM 549 conjugate (red) fluorescent signal. Nuclei were counter-stained using DAPI (blue).
Comparison of fluorescence absorbtion and emission spectra for DyLight？ 488 and Alexa Fluor 488 in PBS, pH7.2. The emission spectra for this DyLight？ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Properties of DyLightTM Fluorescent Dyes. The emission spectra for this DyLightTM conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Western Blot of DyLightTM 488 conjugated anti-GFP antibody to detect GFP control proteins. Lane 1: His-Sumo-GFP. Lane: Molecular Weight. Lane 2: Beta-Galactosidase (negative control). Lane 3: recombinant GFP control protein (000-001-215). Load: 35 ug per lane. Primary antibody: none. Secondary antibody: DyLightTM 488 conjugated anti-GFP goat secondary antibody at 1:5,000. Block: MB-070 for 2 hr at RT. Predicted/Observed size: 27kDa/54kDa, 27kDa for rGFP/~45kDa His-Sumo-GFP. Other band(s): none.
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Western Bot of Goat Anti GFP (GOAT) Antibody DyLightTM 488 Conjugated. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB 210 0500). Lane 2: GFP / HEK293T Whole Cell Lysate Reduced [0.05/10ug]. Lane 3: HEK293T Whole Cell Lysate Reduced (p/n W09 001 GX5) [10ug]. Lane 4: GFP / NIH 3T3 Whole Cell Lysate Reduced [0.05/10ug]. Lane 5: NIH 3T3 Whole Cell Lysate Reduced (p/n W10 000 358) [10ug]. Lane 6: GFP / PC 12 Whole Cell Lysate Reduced [0.05/10ug].Lane 7: PC 12 Whole Cell Lysate Reduced (p/n W12 001 GL9) [10ug]. Secondary Antibody: Goat Anti GFP (GOAT) Antibody DyLightTM 488 Conjugated at 1.0ug/mL overnight at 2 8C. Blocking Buffer: Blocking Buffer for Fluorescent Western Blotting (p/n MB 070) for 1 hour at RT. Expect: 27kDa.
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| メーカー |
品番 |
包装 |
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RKL
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600-141-215
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 法規制 |
毒
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| 保存温度 |
４℃
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