| 別品名 |
GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.
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| 種由来 |
Aequorea victoria
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Chicken
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| Tag情報 |
GFP
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| 形状 |
滅菌済み液状品
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| 参考文献 |
[Pub Med ID]26487778, 27439313, 28065412, 28258213, 28683272, 29249001, 29346754, 29534195, 30033367, 30246807, 30315165, 30775090, 31780329, 32302570, 33125871, 34536353, 34912112, 35030320, 35197635, 35468130+他多数
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot Results of Chicken Anti GFP Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB 210 0500). Lane 2: GFP (p/n 000 001 215) / HeLa WCL (p/n W09 000 364) [0.05ug/10ug]. Lane 3: GFP (p/n 000 001 215) / NIH3T3 WCL (p/n W10 000 371) [0.05ug/10ug]. Lane 4: GFP (p/n 000 001 215) / PC 12 WCL (p/n W120 001 GL9) [0.05ug/10ug]. Lane 5: HeLa WCL (p/n W09 000 364) [10ug]. Lane 6: NIH3T3 WCL (p/n W10 000 371) [10ug]. Lane 7: PC 12 WCL (p/n W120 001 GL9) [10ug]. Primary Antibody: Anti GFP at 1.0ug/mL overnight at 2 8C. Secondary Antibody: Goat Anti Chicken IgG HRP (p/n 603 103 126) at 1:40,000 for 30 mins at RT. Block: MB 070 Buffer for 1hr at RT. Predicted MW: 27kda. Exposure: 2 seconds.
Immunofluorescence Microscopy of Chicken anti-GFP antibody. Tissue: KruppleGAL4 driver line in Drosophila eye disc. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: anti-GFP antibody diluted 1:500 for 2 hr at RT. Secondary antibody: AlexaTM488 conjugated anti-Chicken IgG at 1:300 for 1 hr at RT. Blocking: 5% NGS in PBS with 0.1% Triton X-100 for 15 min. Localization: E2F-1 pS364 is nuclear and occasionally cytoplasmic. Staining: recombinant tau-myc-GFP protein as green fluorescent signal.
Western Blot of Anti-GFP antibody. Lane 1: mouse spleen lysate. Lane 2: mouse spleen lysate spiked with 50 ng of wt GFP. Load: 20 ug per lane. Primary antibody: GFP antibody at 2 ug/ml for 2 hr at room temperature. Secondary antibody: IRDyeTM800 Conjugated Affinity Purified anti-Chicken IgG [H&L] [Goat] MX10 (p/n 603-132-126) at 1:20,000 for 45 min at RT. Block: 5% BSA in PBS 2 hr at room temperature. Predicted/Observed size: 27 kDa for GFP epitope tag. Other band(s): none.
Western Blot of Anti-GFP (CHICKEN) antibody. Lane 1: MW. Lane 2: GFP (p/n 000-001-215) Load: 0.05 ug. Primary antibody: Anti-GFP (CHICKEN) antibody (p/n 600-901-B12) was used at 1:1000 overnight at 4C. Secondary antibody: Anti-Chicken IgG (GOAT) peroxidase conjugated (p/n 603-103-126) secondary antibody was used at 1:40,000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070). Block: 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20C. Predicted/Observed size: 27 kDa for GFP. Other band(s): none.
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Western Blot Results of Chicken Anti GFP Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB 210 0500). Lane 2: GFP (p/n 000 001 215) / HeLa WCL (p/n W09 000 364) [0.05ug/10ug]. Lane 3: GFP (p/n 000 001 215) / NIH3T3 WCL (p/n W10 000 371) [0.05ug/10ug]. Lane 4: GFP (p/n 000 001 215) / PC 12 WCL (p/n W120 001 GL9) [0.05ug/10ug]. Lane 5: HeLa WCL (p/n W09 000 364) [10ug]. Lane 6: NIH3T3 WCL (p/n W10 000 371) [10ug]. Lane 7: PC 12 WCL (p/n W120 001 GL9) [10ug]. Primary Antibody: Anti GFP at 1.0ug/mL overnight at 2 8C. Secondary Antibody: Goat Anti Chicken IgG HRP (p/n 603 103 126) at 1:40,000 for 30 mins at RT. Block: MB 070 Buffer for 1hr at RT. Predicted MW: 27kda. Exposure: 2 seconds.
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| メーカー |
品番 |
包装 |
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RKL
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600-901-B12
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300 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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