| 別品名 |
GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.
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| 種由来 |
Aequorea victoria
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Dot Blot
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| 免疫動物 |
Chicken
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| Tag情報 |
GFP
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| 形状 |
滅菌済み液状品
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| 参考文献 |
[Pub Med ID]22031893, 27525292, 32183906, 26487778, 27439313, 28258213, 29346754, 30315165, 30775090, 32071070, 30246807, 28683272, 29249001, 29534195, 32302570, 30033367, 33125871, 31780329, 27525292, 35468130+他多数
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot of Chicken anti GFP Antibody with Serums. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB 210 0500). Lane 2: GFP/Human Serum (p/n 000 001 215/D314 05) [0.01/0.02uL] [+]. Lane 3: Human Serum (p/n D314 05) [0.02uL] [ ]. Lane 4: GFP/Mouse Serum (p/n 000 001 215/D308 05) [0.01/0.02uL] [+]. Lane 5: Mouse Serum (p/n D308 05) [0.02uL] [ ]. Lane 6: GFP/Rat Serum (p/n 000 001 215/D310 05) [0.01/0.02uL] [+]. Lane 7: Rat Serum (p/n D310 05) [0.02uL] [ ]. Primary antibody: Anti GFP antibody at 1.0ug/mL overnight at 2 8C. Secondary antibody: Donkey Anti Chicken IgG HRP secondary antibody (p/n 603 103 126) at 1:40,000 for 30 min at RT. Blocking Buffer: BlockOut Buffer (p/n MB 073) for 1hr at RT. Predicted/Observed size: ~27kDa for GFP.
Immunofluorescence Microscopy of chicken anti-GFP antibody. Tissue: KruppleGAL4 driver line in Drosophila eye disc. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: anti-GFP antibody diluted 1:500 for 2 hr at RT. Secondary antibody: AlexaTM488 conjugated anti-Chicken IgG at 1:300 for 1 hr at RT. Blocking: 5% NGS in PBS with 0.1% Triton X-100 for 15 min. Staining: recombinant tau-myc-GFP protein as green fluorescent signal.
Western Blot of anti-GFP antibody. Lane 1: mouse spleen lysate (p/n W10-000-T029). Lane 2: mouse spleen lysate spiked with 50 ng of wt GFP (p/n W10-000-T029/000-001-215). Load: 20 μg per lane. Primary antibody: GFP antibody at 2 μg/ml for 2 hr at room temperature. Secondary antibody: IRDye？800 Conjugated Affinity Purified anti-Chicken IgG [H&L] [Goat] MX10 (p/n 603-132-126) at 1:20,000 for 45 min at RT. Block: 5% BSA in PBS 2 hr at room temperature. Predicted/Observed size: 27 kDa for GFP epitope tag. Other band(s): none.
Western Blot of Anti-GFP (CHICKEN) antibody. Lane 1: MW. Lane 2: GFP (p/n 000-001-215). Load: 0.05 ug. Primary antibody: Anti-GFP (CHICKEN) antibody (p/n 600-901-215) was used at 1:1000 overnight at 4C. Secondary antibody: Anti-Chicken IgG (GOAT) peroxidase conjugated antibody (p/n 603-103-126) secondary antibody was used at 1:40,000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070). Block: 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20C. Predicted/Observed size: 27 kDa for GFP. Other band(s): none.
Western Blot Results of Chicken Anti-GFP Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: GFP (p/n 000-001-215) / HeLa WCL (p/n W09-000-364) [0.05ug/10ug]. Lane 3: GFP (p/n 000-001-215) / NIH3T3 WCL (p/n W10-000-371) [0.05ug/10ug]. Lane 4: GFP (p/n 000-001-215) / PC-12 WCL (p/n W120-001-GL9) [0.05ug/10ug]. Lane 5: HeLa WCL (p/n W09-000-364) [10ug]. Lane 6: NIH3T3 WCL (p/n W10-000-371) [10ug]. Lane 7: PC-12 WCL (p/n W120-001-GL9) [10ug]. Primary Antibody: Anti-GFP at 1.0ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Chicken IgG HRP (p/n 603-103-126) at 1:40,000 for 30 mins at RT. Block: MB-070 Buffer for 1hr at RT. Predicted MW: 27kda. Exposure: 2 seconds.
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Western Blot of Chicken anti GFP Antibody with Serums. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB 210 0500). Lane 2: GFP/Human Serum (p/n 000 001 215/D314 05) [0.01/0.02uL] [+]. Lane 3: Human Serum (p/n D314 05) [0.02uL] [ ]. Lane 4: GFP/Mouse Serum (p/n 000 001 215/D308 05) [0.01/0.02uL] [+]. Lane 5: Mouse Serum (p/n D308 05) [0.02uL] [ ]. Lane 6: GFP/Rat Serum (p/n 000 001 215/D310 05) [0.01/0.02uL] [+]. Lane 7: Rat Serum (p/n D310 05) [0.02uL] [ ]. Primary antibody: Anti GFP antibody at 1.0ug/mL overnight at 2 8C. Secondary antibody: Donkey Anti Chicken IgG HRP secondary antibody (p/n 603 103 126) at 1:40,000 for 30 min at RT. Blocking Buffer: BlockOut Buffer (p/n MB 073) for 1hr at RT. Predicted/Observed size: ~27kDa for GFP.
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| メーカー |
品番 |
包装 |
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RKL
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600-901-215S
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25 UL
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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