別品名 |
MGF antibody, Signal Transducer and Activator of Transcription 5A antibody, STAT 5 antibody
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種由来 |
Mouse
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標識物 |
Unlabeled
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精製度 |
Ig fraction - Protein A
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry Immuno Fluorescence
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免疫動物 |
Mouse
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抗体クラス |
IgG1κ
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クローン |
5F6.F1
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交差種 |
Human Mouse
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翻訳後修飾 |
リン酸化
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GENE ID |
20850
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Accession No.(Gene/Protein) |
P42230
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Gene Symbol |
STAT5A
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形状 |
滅菌済み液状品
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参考文献 |
Engblom,D., Kornfeld,J.W., Schwake,L., Tronche,F., Reimann,A., Beug,H., Hennighausen,L., Moriggl,R. and Schutz,G. (2007) Direct glucocorticoid receptor-Stat5 interaction in hepatocytes controls body size and maturation-related gene expression. Genes Dev. 21 (10), 1157-1162. Baugh,J.E. Jr., Floyd,Z.E. and Stephens,J.M. (2007) The modulation of STAT5A/GR complexes during fat cell differentiation and in mature adipocytes. Obesity (Silver Spring) 15 (3), 583-590. Laurence,A., Tato,C.M., Davidson,T.S., Kanno,Y., Chen,Z., Yao,Z., Blank,R.B., Meylan,F., Siegel,R., Hennighausen,L., Shevach,E.M. and O'Shea,J.J. (2007) Interleukin-2 signaling via STAT5 constrains T helper 17 cell generation. Immunity 26 (3), 371-381.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunohistochemistry using Rockland Immunochemicals Anti STAT5 pY694 monoclonal antibody shows detection of phosphorylated STAT5 pY694 in human breast tissue (40X). The antibody was used a dilution to 20 ug/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. No pre treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.
Immunofluorescent STED Microscopy of Anti-Stat5 pY694 (MOUSE) Monoclonal Antibody in 3T3 cells. Anti-Stat5 pY694 at 1:500 detecting Stat5 in 3T3 cells. Staining: Stat5 pY694 protein [Red]. Tubulin [Green].
Western blot using Rockland's Protein A purified Mouse Monoclonal anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4 C. For detection DyLightTM800 conjugated Gt-a-Mouse IgG (p/n 610-145-002) was used at a 1:20,000 dilution for 30 min at room temperature followed by visualization using a VersaDocTM MP 4000 imaging system (Bio-Rad).
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Immunohistochemistry using Rockland Immunochemicals Anti STAT5 pY694 monoclonal antibody shows detection of phosphorylated STAT5 pY694 in human breast tissue (40X). The antibody was used a dilution to 20 ug/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. No pre treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.
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メーカー |
品番 |
包装 |
RKL
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200-301-B18
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1 MG
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※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
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保存温度 |
-20℃
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