| 別品名 |
MGF antibody, Signal Transducer and Activator of Transcription 5A antibody, STAT 5 antibody
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| 種由来 |
Mouse
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein A
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Immuno Fluorescence
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| 免疫動物 |
Mouse
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| 抗体クラス |
IgG1κ
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| クローン |
5F6.F1
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| 交差種 |
Human
Mouse
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| 翻訳後修飾 |
リン酸化
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| GENE ID |
20850
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| Accession No.(Gene/Protein) |
P42230
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| Gene Symbol |
STAT5A
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| 形状 |
滅菌済み液状品
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| 参考文献 |
Engblom,D., Kornfeld,J.W., Schwake,L., Tronche,F., Reimann,A., Beug,H., Hennighausen,L., Moriggl,R. and Schutz,G. (2007) Direct glucocorticoid receptor-Stat5 interaction in hepatocytes controls body size and maturation-related gene expression. Genes Dev. 21 (10), 1157-1162. Baugh,J.E. Jr., Floyd,Z.E. and Stephens,J.M. (2007) The modulation of STAT5A/GR complexes during fat cell differentiation and in mature adipocytes. Obesity (Silver Spring) 15 (3), 583-590. Laurence,A., Tato,C.M., Davidson,T.S., Kanno,Y., Chen,Z., Yao,Z., Blank,R.B., Meylan,F., Siegel,R., Hennighausen,L., Shevach,E.M. and O'Shea,J.J. (2007) Interleukin-2 signaling via STAT5 constrains T helper 17 cell generation. Immunity 26 (3), 371-381.
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunohistochemistry of Monoclonal Anti Stat5 pY694 Antibody. Tissue: human breast tissue (40X). Fixation: formalin fixed paraffin embedded (FFPE). Antigen retrieval: steam sections in 0.1 M sodium citrate buffer, pH 6, for 20 min. Rinse with 1XTBST. Primary antibody: Anti Stat5pY694 at 20 ug/mL. Localization: breast epithelium with moderate nuclear staining. Staining: Stat5 pY694 as precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.
Immunofluorescent STED Microscopy of Anti-Stat5 pY694 (MOUSE) Monoclonal Antibody in 3T3 cells. Anti-Stat5 pY694 at 1:500 detecting Stat5 in 3T3 cells. Staining: Stat5 pY694 protein [Red]. Tubulin [Green].
Western blot using Rockland's Protein A purified Mouse Monoclonal anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4 C. For detection DyLightTM800 conjugated Gt-a-Mouse IgG (p/n 610-145-002) was used at a 1:20,000 dilution for 30 min at room temperature followed by visualization using a VersaDocTM MP 4000 imaging system (Bio-Rad).
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Immunohistochemistry of Monoclonal Anti Stat5 pY694 Antibody. Tissue: human breast tissue (40X). Fixation: formalin fixed paraffin embedded (FFPE). Antigen retrieval: steam sections in 0.1 M sodium citrate buffer, pH 6, for 20 min. Rinse with 1XTBST. Primary antibody: Anti Stat5pY694 at 20 ug/mL. Localization: breast epithelium with moderate nuclear staining. Staining: Stat5 pY694 as precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.
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| メーカー |
品番 |
包装 |
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RKL
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200-301-A45
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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