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Rockland Antibody 200-301-964 has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The antibody was serially diluted and tested at a range of concentrations on at least 22 different human formalin-fixed, paraffin archival tissues, and positive and negative tissues were scored and compared to the published literature on the expression and function of the gene. A representative image from positively stained small intestine shows the localization of the anti Pdcd4 antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Image provided courtesy of LifeSpan Biosciences, Seattle, WA
Western blot using Rockland's Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody shows detection of phosphorylated Pdcd4 (indicated by arrowhead at ~62 kDa) in NIH-3T3 cells (p/n W10-001-377) after 5 min treatment with 30 ng/mL PDGF [lane 2]. No reactivity is seen for (non-phosphorylated) in unstimulated NIH 3T3 cells (p/n W10-000-358) [lane 1]. The membrane was probed with the primary antibody at a 1:2,000 dilution, overnight at 4° C. For detection HRP conjugated Rb-a-Mouse IgG (p/n 610-4302) was used at a 1:20,000 dilution in blocking buffer (p/n MB-070) for 1 h at 4° C followed by visualization using a BiospectrumR imaging system (UVP).
Western blot using Rockland Immunochemicals Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody against recombinant PDCD4 protein. Membrane was blocked in 1% BSA-TBS-T for 30 min RT and probed with 1° Ab Ms-A-Pdcd4pS457 1:1000 (o/n 4°C in 1% BSA-TBS-T) followed by 2° Ab Peroxidase Conjugated Rabbit anti-Ms CUST10M Lot# 20121 at 1:40,000 in MB-070 30 min RT. Bands at ~62 kD and ~32 kD were detected.
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Rockland Antibody 200-301-964 has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The antibody was serially diluted and tested at a range of concentrations on at least 22 different human formalin-fixed, paraffin archival tissues, and positive and negative tissues were scored and compared to the published literature on the expression and function of the gene. A representative image from positively stained small intestine shows the localization of the anti Pdcd4 antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Image provided courtesy of LifeSpan Biosciences, Seattle, WA
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| 別品名 |
mouse anti-Pdcd4 pS457 Antibody, Death up-regulated gene protein antibody, Dug antibody, H731 antibody, Ma3 antibody, Neoplastic transformation inhibitor antibody, Neoplastic transformation inhibitor protein antibody, Nuclear antigen H731 antibody
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| 交差種 |
Human
Mouse
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
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| 免疫動物 |
Mouse
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| クローン |
9G6
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| 抗体クラス |
IgG1κ
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein A
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| 翻訳後修飾 |
リン酸化
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| GENE ID |
27250
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| Accession No.(Gene/Protein) |
21735596, Q53EL6
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| Gene Symbol |
PDCD4
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| 参考文献 |
Jansen AP, Camalier CE, Colburn NH (2005) Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis. Cancer Res. 65(14):6034-6041. Palamarchuk A, Efanov A, Maximov V, Aqeilan RI, Croce CM, Pekarsky Y (2005) Akt phosphorylates and regulates Pdcd4 tumor suppressor protein. Cancer Res. 65 (24):11282-11286. Tzu-Hsuan Huang, GB Loeb, R Hsu, A Heidersbach, A Brincat, D Horiuchi, RJ Lebbink, YY Mo, A Goga, MT McManus. Up-regulation of miR-21 by HER2/neu Signaling Promotes Cell Invasion J. Biol. Chem. 2009 284: 18515-18524. First Published on May 6, 2009, doi:10.1074/jbc.M109.006676
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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200-301-964S
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25 UL
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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