| 別品名 |
FLAGTM tag, Enterokinase Cleavage Site (ECS), DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein A
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Mouse
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| 抗体クラス |
IgG2aκ
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| クローン |
29E4.G7
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| Tag情報 |
FLAG
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| 形状 |
滅菌済み液状品
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| 参考文献 |
[Pub Med ID]25739390, 26966178, 27485701, 28264028, 28700677, 25767741, 32886017, 32286293
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Twenty four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 uL. Samples were diluted 10 fold, transferred to nitrocellulose membrane and blotted using Mab anti FLAGTM antibody. Personal Communication: A. Morrison and B. Kloss, NYCOMPS, New York, NY.
Monoclonal Antibody to detect FLAGTM conjugated proteins detects both C terminal linked and N terminal linked FLAGTM tagged recombinant proteins by western blot.
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Twenty four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 uL. Samples were diluted 10 fold, transferred to nitrocellulose membrane and blotted using Mab anti FLAGTM antibody. Personal Communication: A. Morrison and B. Kloss, NYCOMPS, New York, NY.
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| メーカー |
品番 |
包装 |
|
RKL
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200-301-383S
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25 UL
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※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
|
| 保存温度 |
-２０℃
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