Anti FLAG^(R) DYKDDDDK  
FLAG (商標名)

Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 μL. Samples were diluted 10-fold, transferred to nitrocellulose membrane and blotted using Mab-anti-FLAG^TM antibody. Personal Communication: A. Morrison and B. Kloss, NYCOMPS, New York, NY.

Monoclonal Antibody to detect FLAG^TM conjugated proteins detects both C terminal linked and N terminal linked FLAG^TM tagged recombinant proteins by western blot.

Immunocytochemical analysis of signaling pathways involved in NGF-induced p11 production. (A) Confocal images of p11 and HA- or TrkA-like immunofluorescence in PC12nnr5 cells expressing wild-type (WT), Y499F-TrkA, Y760F-TrkA, or Y794F-TrkA. First and second columns represent p11- and HA- or Flag-like (p/n 200-301-383) immunofluorescence images, respectively; third, merge of DIC and fluorescence images. HA-like fluorescence was observed in cells expressing WT, Y760F-TrkA, and Y794F-TrkA, whereas TrkA-like fluorescence was observed in cells expressing Y760F-TrkA. (B) p11-like fluorescence is plotted against those of HA- (WT, Y499F, and Y794F) or TrkA-like (Y760F) fluorescence observed in the same cells. Linear regression lines are as follows―WT:？y？= −0.634 + 0.810x, correlation coefficient？r？= 0.823; Y499F:？y？= −5.595 + 0.621x, r？= 0.924; Y760F:？y？= −5.594 + 0.901x, r？= 0.929; Y794F:？y？= −3.623 + 0.541x, r？= 0.888. (C) Summary of p11-like IR material in PC12nnr5 cells expressing WT, Y499F-TrkA, Y760F-TrkA, or Y794F-TrkA. P11-like IR material was measured over the whole cell area including protrusions or neurites. Data are mean ± SEM; Kruskal？Wallis one-way analysis of variance on ranks (WT:？n=38 from three culture dishes; Y499F:？n=33 from two; Y760F:？n=38 from three; Y794F:？n=38 from 3). Bars are 10 μm. Abbreviations: DIC, differential interference contrast; HA, hemagglutinin; IR, immunoreactive; NGF, nerve growth factor; PC, pheochromocytoma. Fig 5. PMID: 32886017

Inhibition of ATR activation inhibits the NS1-induced G2-phase arrest of CD36+？EPCs.(A) Cell cycle analysis. CD36+？EPCs were treated with VE821 at 3 h prior to NS1-expressing lentivirus transduction or mock transduction. After 48 h, cells were collected, and the cell cycle phase of NS1-expressing cells (selected by staining with anti-Flag) was examined by flow cytometry. (B) Statistical analyses. The percentage of cells treated with DMSO or VE821 that were at G1-, S-, and G2-phase is depicted in color. The numbers shown are the percentages of the cells at G2-phase, and are statistically compared as indicated. ** P<0.01. Fig 8. PMID: 28264028