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| 種由来 |
Human
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| 励起波長 |
485
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| 蛍光波長 |
528
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| Tag情報 |
6-His tag
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| 概要 |
The Autotaxin Inhibitor Screening Kit utilizes purified autotaxin (ATX) and the autotaxin substrate FS-3 which fluoresces upon cleavage. The assay is performed in a 96 well plate and quantifies the autotaxin activity in the presence of the user's samples by measuring changes in fluorescence over time.Sample Type: Inhibitors against ATXSample Volume: 10 AμL/ well of your sample compoundsSample Considerations: Some organic solvents interfere with the assay (see technical data sheet).Product BackgroundThe Autotaxin Inhibitor Screening Kit developed by Echelon Biosciences Inc. uses the fluorogenic autotaxin substrate FS-3. FS-3 is an LPC analogue that is conjugated with both a fluorophore and a quencher. In its native state the quencher interferes with the fluorophorea？？s fluorescence however once autotaxin cleaves FS-3 the fluorophore becomes liberated from the quencher resulting in increased fluorescence.Percent autotaxin inhibition is determined by referencing no inhibitor controls. The autotaxin inhibitor BrP-LPA is supplied to positively control for autotaxin inhibition. The assay is resistant to modest concentrations of DMSO and is performed in a continuous assay format which has the advantage of being less susceptible to error arising from mis-timing the addition of components to different sample wells.Product Keywords: ATX LPA FS-3 LPC lysophospholipase D BrP-LPA Powered by Bioz See more details on BiozNote to Purchaser:A？Echelon Biosciences products are sold for research and development purposes only and are not to be incorporated into products for resale without written permission from Echelon Biosciences. The compound FS-3 and its use in assaying for Lysophospholipase D activity are covered by Echelon Biosciences Inc. US patent 7 989 663. The purchase of this product includes a limited non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. For inquiries email busdev@echelon-inc.com
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| 構成内容 |
・#E-4000, Autotaxin enzyme
・#K-4202, Fluorescent autotaxin substrate (FS-3)
・#K-4102, Fluorescein dye
・#K-4104, Buffer A (fluorescein dye suspension buffer)
・#K-4106, Buffer C (concentrated reaction buffer)
・#K-4107, Buffer D (protein stabilizer)
・#K-4204, Inhibitor positive control (BrP-LPA)
・2 X 96 well assay plates
・2 X Plate sealer
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| メーカー |
品番 |
包装 |
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ECL
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K-4200
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1 KIT
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 法規制 |
カルタヘナ
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| 保存温度 |
-２０℃
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