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| 種由来 |
Human
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| 励起波長 |
485
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| 蛍光波長 |
528
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| Tag情報 |
6-His tag
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| 発現系 |
昆虫細胞
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| 概要 |
The Autotaxin Activity Assay gives a quantitative measurement of autotaxin (ATX) activity in biological samples by measuring changes in fluorescence over time.Sample Volume: 400 AμL / sample (50 AμL per well 8 wells per sample)Sample Number: 10 samples (in duplicate) / 96 well assaySample Type: Currently this kit has been tested for the quantification of autotaxin activity in serum plasma ovarian ascites and cell culture media.Product BackgroundThe Autotaxin Activity Assay developed by Echelon Biosciences Inc. utilizes the fluorogenic autotaxin substrate FS-3. FS-3 is an LPC analogue that is conjugated with both a fluorophore and a quencher. In its native state the quencher interferes with the fluorophorea？？s fluorescence however once autotaxin cleaves FS-3 the fluorophore becomes liberated from the quencher resulting in increased fluorescence.This assay gives a quantitative measurement of autotaxin activity in biological samples by measuring changes in fluorescence over time. Because the assay is not end point based it has the advantage of being less susceptible to error arising from mistiming the addition of components to different sample wells. The final readout is in quantitative units that correct for inconsistencies between different users equipment.Note to Purchaser:A？Echelon Biosciences products are sold for research and development purposes only and are not to be incorporated into products for resale without written permission from Echelon Biosciences. The compound FS-3 and its use in assaying for Lysophospholipase D activity are covered by Echelon Biosciences Inc. US patent 7 989 663. The purchase of this product includes a limited non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. For inquiries email busdev@echelon-inc.comProduct Keywords: ATX LPA FS-3 LPC lysophospholipase D Powered by Bioz See more details on Bioz
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| 構成内容 |
・#E-4000, Autotaxin enzyme
・#L-2000, Fluorescent autotaxin substrate (FS-3)
・#K-4102, Fluorescein dye (for standard curve)
・#K-4103, Lysophosphatidylcholine (LPC)
・#K-4104, Buffer A (fluorescein dye suspension buffer)
・#K-4105, Buffer B (lysophosphatidylcholine suspension buffer)
・#K-4106, Buffer C (concentrated reaction buffer)
・#K-4107, Buffer D (protein stabilizer)
・96 well assay plate
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| メーカー |
品番 |
包装 |
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ECL
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K-4100
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1 KIT
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 法規制 |
安・カルタヘナ
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| 保存温度 |
-２０℃
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