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Co-IP of MCM complex via pulldown of MCM6 using 5 μg of monoclonal anti-MCM6 IgG1 (Proteintech: 67989-1-Ig) and anti-mouse IgG1 magnetic VHH agarose (mIG1ma). 5 μg of each IgG was spiked into HEK293T cell lysate derived from 1x10^7 cells. All subunits of the 600 kDa hetero-hexameric complex are successfully precipitated, as shown in a Coomassie blue stained SDS-gel and by Western blot analysis using subunit specific antibodies. Apparent molecular weights are provided. For input (IN) and flowthrough (FT) fractions, 1% was loaded, respectively. For bound (B) fraction and bound fraction of isotype control antibody (BISO PTG: 66360-1-Ig), 25% was loaded. Product codes for Proteintech antibodies are provided in parentheses. Compared to Protein A magnetic agarose, mIG1ma shows a superior precipitation of the MCM complex.
IP of PCNA by anti-mouse IgG1 magnetic VHH agarose (mIG1ma) using the PCNA Monoclonal antibody (PTG: 60097-1-Ig). As control, a IgG1 isotype control antibody (Proteintech: 66360-1-Ig) was used (BISO). 5 μg of each IgG was spiked into HEK293T cell lysate derived from 0.5x10^7 cells. 1% of input (IN) and flow through (FT) and 25% of bound (B) fraction was loaded onto an SDS-PAGE gel. For Western blot analysis PCNA was detected using a polyclonal rabbit IgG (Proteintech: 10205-2-AP) (1:5000) and an HRP-labeled goat anti-rabbit secondary (Proteintech: RGAR001). Compared to Protein A and G magnetic beads, mIG1ma shows a superior precipitation and allows for a cleaner detection of PCNA.
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Co-IP of MCM complex via pulldown of MCM6 using 5 μg of monoclonal anti-MCM6 IgG1 (Proteintech: 67989-1-Ig) and anti-mouse IgG1 magnetic VHH agarose (mIG1ma). 5 μg of each IgG was spiked into HEK293T cell lysate derived from 1x10^7 cells. All subunits of the 600 kDa hetero-hexameric complex are successfully precipitated, as shown in a Coomassie blue stained SDS-gel and by Western blot analysis using subunit specific antibodies. Apparent molecular weights are provided. For input (IN) and flowthrough (FT) fractions, 1% was loaded, respectively. For bound (B) fraction and bound fraction of isotype control antibody (BISO PTG: 66360-1-Ig), 25% was loaded. Product codes for Proteintech antibodies are provided in parentheses. Compared to Protein A magnetic agarose, mIG1ma shows a superior precipitation of the MCM complex.
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| 別品名 |
Mouse IP, IgG IP, IgG Trap, Secondary VHH, VHH, Nanobody
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| 別包装 |
あり
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| 種由来 |
Mouse
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| 交差種 |
Mouse
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| 適用 |
Immunoprecipitation
Co-Immunoprecipitation
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| 免疫動物 |
Alpaca
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| 標識物 |
Magnetic Particle
Agarose
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| その他 |
[備考]旧Chromotek（クロモテック、ドイツ）社商品。2020年10月よりProteintech Group（プロテインテック、米国）社ブランドの一部になりました。
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| メーカー |
品番 |
包装 |
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PGI
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MIG1MA-5
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5 ML
[1ml*5]
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 法規制 |
安
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| 保存温度 |
４℃禁凍結
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