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IF staining using PK10034 (Five color TSA Detect Kit for Rabbit/Mouse Primary Antibody). FFPE-rat brain tissue was stained with Lamin B1 monoclonal antibody (Cat.NO. 66095-1-Ig) at 1:2000 followed by detection with Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody (H+L). CoraLitePlus 555-Tyramide Reagent was used for signal development (yellow). The slide was then conducted HIER in pH 9.0 Tris-EDTA buffer followed by staining with GFAP monoclonal antibody (60190-1-Ig, 1:20000). CoraLitePlus 488-Tyramide Reagent were used for signal development (green). Similarly, IBA1 recombinant antibody (81728-1-RR, 1:2000) TDP-43 recombinant antibody (80002-1-RR, 1:3200) were stained subsequently. CoraLitePlus 594-Tyramide Reagent (red) and CoraLitePlus 647-Tyramide Reagent (purple) were used for signal development respectively. Finally, cell nucleus (blue) were stained with DAPI. The signal from each channel was captured and merged.
IF staining using using PK10034 (Five color TSA Detect Kit for Rabbit/Mouse Primary Antibody). FFPE-human tonsillitis tissue was stained with CD23 monoclonal antibody (60208-2-Ig) at 1:8000 followed by detection with Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody (H+L). CoraLitePlus 555-Tyramide Reagent was used for signal development (yellow). The slide was then conducted HIER in pH 9.0 Tris-EDTA buffer followed by staining with CD3 monoclonal antibody (60181-1-Ig, 1:10000). CoraLitePlus 488-Tyramide Reagent were used for signal development (green). Similarly, CD8a monoclonal antibody (66868-1-Ig, 1:20000) and CD138 monoclonal antibody (67155-1-Ig, 1:10000) were stained subsequently. CoraLitePlus 594-Tyramide Reagent (red) and CoraLitePlus 647-Tyramide Reagent (purple) were used for signal development respectively. Finally, cell nucleus (blue) were stained with DAPI. The signal from each channel was captured and merged.
IF staining using using PK10034 (Five color TSA Detect Kit for Rabbit/Mouse Primary Antibody). FFPE-human tonsillitis tissue was stained with CD23 monoclonal antibody (60208-2-Ig) at 1:8000 followed by detection with Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody (H+L). CoraLitePlus 555-Tyramide Reagent was used for signal development (yellow). The slide was then conducted HIER in pH 9.0 Tris-EDTA buffer followed by staining with CD3 monoclonal antibody (60181-1-Ig, 1:10000). CoraLitePlus 488-Tyramide Reagent were used for signal development (green). Similarly, CD8a monoclonal antibody (66868-1-Ig, 1:20000) and CD138 monoclonal antibody (67155-1-Ig, 1:10000) were stained subsequently. CoraLitePlus 594-Tyramide Reagent (red) and CoraLitePlus 647-Tyramide Reagent (purple) were used for signal development respectively. Finally, cell nucleus (blue) were stained with DAPI.
IF staining using PK10034 (Five color TSA Detect Kit for Rabbit/Mouse Primary Antibody). FFPE-rat brain tissue was stained with Lamin B1 monoclonal antibody (Cat.NO. 66095-1-Ig) at 1:2000 followed by detection with Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody (H+L). CoraLitePlus 555-Tyramide Reagent was used for signal development (yellow). The slide was then conducted HIER in pH 9.0 Tris-EDTA buffer followed by staining with GFAP monoclonal antibody (60190-1-Ig, 1:20000). CoraLitePlus 488-Tyramide Reagent were used for signal development (green). Similarly, IBA1 recombinant antibody (81728-1-RR, 1:2000) TDP-43 recombinant antibody (80002-1-RR, 1:3200) were stained subsequently. CoraLitePlus 594-Tyramide Reagent (red) and CoraLitePlus 647-Tyramide Reagent (purple) were used for signal development respectively. Finally, cell nucleus (blue) were stained with DAPI.
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| 標識物 |
CoraLite(R) Plus 594
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| 使用目的 |
This kit provides enough material to perform 5-color staining of 50 tissue sections or cell slides. It contains four different TSA dyes, nuclear DAPI stain, a Poly-HRP-Goat Anti-Rabbit/Mouse Universal Secondary antibody, amplification buffer, blocking/dilution buffer, and quenching buffer.
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| 構成内容 |
● Quenching Buffer(30 mL)
● Blocking/Dilution Buffer(100 mL)
● Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse？Universal Recombinant Secondary Antibody (H+L)(20 mL)
● CoraLitePlus 488-Tyramide？Reagent(100 uL)
● CoraLitePlus 555-Tyramide？Reagent(100 uL)
● CoraLitePlus 594-Tyramide？Reagent(100 uL)
● CoraLitePlus 647-Tyramide？Reagent(100 uL)
● Amplification Buffer(30 mL)
● DAPI Nuclear Stain(5 mL)
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| メーカー |
品番 |
包装 |
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PGI
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PK10034
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50 TEST
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※表示価格について
| 当社在庫 |
なし
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| 入荷予定 |
あり
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| 納期目安 |
発注済・入荷予定
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| 法規制 |
安・化管・危
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| 保存温度 |
４℃
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