Anti p95/NBS1 NBN  

Immunohistochemical analysis of paraffin-embedded Rat kidney, using the Antibody at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded Rat heart, using the Antibody at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using the Antibody at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using the Antibody at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded Mouse heart, using the Antibody at 1:100 dilution.

Immunofluorescent analysis using the Antibody at 1:50 dilution.

Immunohistochemical analysis of paraffin-embedded rat heart, using p95/NBS1 Antibody.

Figure 1. Western blot analysis of NBN using anti-NBN antibody (M00732).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NBN antigen affinity purified monoclonal antibody (Catalog # M00732) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NBN at approximately 95 kDa. The expected band size for NBN is at 85 kDa.