Anti His-Tag, Fab-Trap^(R) Magnetic Agarose, recombinant Fab  

IP using His Fab-Trap Magnetic Agarose (hfma) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).

His Fab-Trap Magnetic Agarose (hfma) was used for the Co-IP of AurKinB-His fusion protein and interacting partners from untransfected (mock) and transfected HEK293T cells. Multiplexed WB was done on Input (I), Flow-Through (F), and Bound (B) fractions of the IP. Detection was completed using His-tag Monoclonal Antibody (66005-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit for Mouse IgG (KFA023), CDC37 Monoclonal Antibody (66420-1-Ig) labeled with FLexAble CoraLite Plus 750 Kit for Mouse IgG2a (KFA044), and HSP90AB1 Monoclonal Antibody (67450-1-Ig) labeled with FlexAble CoraLite Plus 488 Kit for Mouse IgG2b (KFA061).

IP of GFP-His fusion protein from transfected HEK293T cell using His Fab-Trap Magnetic Agarose (hfma) followed by a two-step elution with His-Peptide (hp). I: Input, F: Flow-Through, E1: 1st elution, E2: 2nd elution, R: Residual.